ACUITY Biotin Free Polymer Detection HRP System 50 Test AEC (SIGNET)_详细资料

产品信息

靶点：来电咨询浓度： 1mg/1ml 应用范围：科研使用宿主： Goat，Rabbit，Mouse 适应物种：人，大鼠，小鼠，兔标记物：见说明书克隆性：多克隆保存条件： -20℃保存形态：液态/粉状

ACUITY Biotin Free Polymer Detection HRP System 50 Test AEC (SIGNET)详细信息：

Description ACUITY Biotin Free Polymer Detection HRP System, AEC, 50 Tests
(Formerly Signet Catalog No.: 2600)

Intended Use **Research Use Only (RUO)**

This product is sold for laboratory research use only, not for human or in-vivo use.

Form ACUITY Biotin Free Polymer Detection HRP System 50 Test AEC (SIGNET)Detection Kit

Specificity Signets ACUITY Detection system is a sensitive biotin-free polymer detection kit that is compatible with antibodies raised in mouse, rat and rabbit. ACUITY contains a horseradish Peroxidase labeled polymer and special blocking solutions to eliminate possible nonspecific background staining.

Kit Contains:

Serum Block, 5 mL

Special Block, 5 mL

HRP Polymer Link, 5 mL

AEC Substrate Buffer, 24 mL

AEC Chromogen, 1 mL

Hydrogen Peroxide, 5 mL

Suggested Working Dilution

ACUITY Biotin Free Polymer Detection HRP System 50 Test AEC (SIGNET) Protocol:
1. Tissue preparation: Deparaffinize and rehydrate according to the established protocols.
2. Blocking step-1: Incubate with H₂0₂ at RT for 15 minutes. Rinse with PBS.
3. Blocking step-2: Apply the Serum Block reagent for 15 minutes
4. Primary antibody: Apply the primary antibody for 30 minutes
5. Blocking step-3: Apply the Special Block reagent for 20 minutes then rinse with PBS (5min/2X).
6. Polymer Link: Apply the Polymer reagent for 30 minutes then rinse with PBS (5min/2X).
7. Chromogen step: Apply AEC Working Solution for 3-5 minutes then rinse with DI water (see AEC preparation guidelines below).
8. Counter stain & mount.

AEC Working Solution* Preparation
Buffer + Chromogen = Working Solution
5 mL + 0.10 mL = 5mL
10 mL + 0.20 mL = 10mL
24 mL + 0.48 mL = 24mL

Notes *Discard unused AEC working solution at the end of the run.

Storage Store between 2-8°C.

Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval.

ACUITY Biotin Free Polymer Detection HRP System 50 Test AEC (SIGNET)

xyA090Hu   巨噬细胞集落刺激因子(MCSF)ELISA试剂盒   检测范围
xyA091Hu   巨噬细胞来源趋化因子(MDC)ELISA试剂盒   检测范围
xyA102Hu   基质金属蛋白酶7(MMP7)ELISA试剂盒   检测范围
xyA103Hu   基质金属蛋白酶8(MMP8)ELISA试剂盒   检测范围
xyA105Hu   神经生长因子(NGF)ELISA试剂盒   检测范围
xyA106Hu   神经营养因子3(NT3)ELISA试剂盒   检测范围
xyA107Hu   神经营养因子4(NT4)ELISA试剂盒   检测范围
xyA108Hu   骨保护素(OPG)ELISA试剂盒   检测范围
xyA110Hu   抑瘤素M(OSM)ELISA试剂盒   检测范围
xyA114Hu   胎盘生长因子(PLGF)ELISA试剂盒   检测范围
xyA116Hu   调节激活正常T-细胞表达分泌因子(RANTES)ELISA试剂盒   检测范围
xyA118Hu   CD30配体(CD30L)ELISA试剂盒   检测范围
xyA119Hu   CD40配体(CD40L)ELISA试剂盒   检测范围
xyA120Hu   干细胞因子(SCF)ELISA试剂盒   检测范围
xyA121Hu   干细胞因子受体(SCFR)ELISA试剂盒   检测范围
xyA122Hu   基质细胞衍生因子1(SDF1)ELISA试剂盒   检测范围
xyA123Hu   转化生长因子α(TGFα)ELISA试剂盒   检测范围
xyA124Hu   转化生长因子β1(TGFβ1)ELISA试剂盒   检测范围
xyA125Hu   免疫球蛋白样EGF样域酪氨酸激酶1(Tie1)ELISA试剂盒   检测范围
xyA126Hu   内皮细胞TEK酪氨酸激酶(Tie2)ELISA试剂盒   检测范围
xyA128Hu   组织金属蛋白酶YZ因子2(TIMP2)ELISA试剂盒   检测范围
xyA129Hu   组织金属蛋白酶YZ因子3(TIMP3)ELISA试剂盒   检测范围
xyA130Hu   组织金属蛋白酶YZ因子4(TIMP4)ELISA试剂盒   检测范围
xyA133Hu   肿瘤坏死因子α(TNFα)ELISA试剂盒   检测范围
xyA134Hu   肿瘤坏死因子β(TNFβ)ELISA试剂盒   检测范围
xyA135Hu   血小板生成素(TPO)ELISA试剂盒   检测范围
xyA140Hu   尿激酶型纤溶酶原激活因子(uPA)ELISA试剂盒   检测范围
xyA141Hu   尿激酶型纤溶酶原激活因子受体(uPAR)ELISA试剂盒   检测范围
xyA142Hu   细胞间粘附分子5(ICAM5)ELISA试剂盒   检测范围
xyB851Hu   血管内皮生长因子121(VEGF121)ELISA试剂盒   检测范围
xyA144Hu   血管内皮生长因子B(VEGFB)ELISA试剂盒   检测范围
xyA145Hu   血管内皮生长因子C(VEGFC)ELISA试剂盒   检测范围
xyA146Hu   血管内皮生长因子D(VEGFD)ELISA试剂盒   检测范围
xyA150Hu   癌胚抗原(HZA)ELISA试剂盒   检测范围
xyA151Hu   激肽释放酶3(KLK3)ELISA试剂盒   检测范围
xyA154Hu   糖抗原125(CA125)ELISA试剂盒   检测范围
xyA155Hu   糖抗原15-3(CA15-3)ELISA试剂盒   检测范围
xyA156Hu   糖抗原19-9(CA19-9)ELISA试剂盒   检测范围
xyA159Hu   鳞状细胞癌抗原2(SCCA2)ELISA试剂盒   检测范围
xyA163Hu   组织多肽抗原(TPA)ELISA试剂盒   检测范围
xyA165Hu   胃蛋白酶原A(PGA)ELISA试剂盒   检测范围

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Description		ACUITY Biotin Free Polymer Detection HRP System, AEC, 50 Tests (Formerly Signet Catalog No.: 2600)
Intended Use		Research Use Only (RUO) This product is sold for laboratory research use only, not for human or in-vivo use.
Form		ACUITY Biotin Free Polymer Detection HRP System 50 Test AEC (SIGNET)Detection Kit
Specificity		Signets ACUITY Detection system is a sensitive biotin-free polymer detection kit that is compatible with antibodies raised in mouse, rat and rabbit. ACUITY contains a horseradish Peroxidase labeled polymer and special blocking solutions to eliminate possible nonspecific background staining. Kit Contains: Serum Block, 5 mL Special Block, 5 mL HRP Polymer Link, 5 mL AEC Substrate Buffer, 24 mL AEC Chromogen, 1 mL Hydrogen Peroxide, 5 mL
Suggested Working Dilution		ACUITY Biotin Free Polymer Detection HRP System 50 Test AEC (SIGNET) Protocol: 1. Tissue preparation: Deparaffinize and rehydrate according to the established protocols. 2. Blocking step-1: Incubate with H₂0₂ at RT for 15 minutes. Rinse with PBS. 3. Blocking step-2: Apply the Serum Block reagent for 15 minutes 4. Primary antibody: Apply the primary antibody for 30 minutes 5. Blocking step-3: Apply the Special Block reagent for 20 minutes then rinse with PBS (5min/2X). 6. Polymer Link: Apply the Polymer reagent for 30 minutes then rinse with PBS (5min/2X). 7. Chromogen step: Apply AEC Working Solution for 3-5 minutes then rinse with DI water (see AEC preparation guidelines below). 8. Counter stain & mount. *AEC Working Solution Preparation** Buffer + Chromogen = Working Solution 5 mL + 0.10 mL = 5mL 10 mL + 0.20 mL = 10mL 24 mL + 0.48 mL = 24mL
Notes		*Discard unused AEC working solution at the end of the run.
Storage		Store between 2-8°C.
Warranty/Conditions		Covance products may not be resold or modified for resale without prior written approval.