Retrieve-All Antigen Unmasking System 1: Universal, 10x (SIGNET)_详细资料

产品信息

靶点：来电咨询浓度： 1mg/1ml 应用范围：科研使用宿主： Goat，Rabbit，Mouse 适应物种：人，大鼠，小鼠，兔标记物：见说明书克隆性：多克隆保存条件： -20℃保存形态：液态/粉状

Retrieve-All Antigen Unmasking System 1: Universal, 10x (SIGNET)保存于运输说明：

Store at room temperature

详细信息：

Description Retrieve-All Antigen Unmasking System 1: Universal (pH 8); 10x
(Formerly Signet Catalog No.: 1914)

Intended Use **Research Use Only (RUO)**

This product is sold for laboratory research use only, not for human or in-vivo use.

Form Retrieve-All Antigen Unmasking System 1: Universal, 10x (SIGNET)Antigen Unmasking Solution

Specificity Retrieve-All is an antigen un-masking solution available in the following three pH formulas for use in heat induced un-masking procedures:

Retrieve-All 1 (Universal pH 8)
Retrieve-All 2 (Basic pH 10)
Retrieve-All 3 (Acidic pH 4.8)

Suggested Working Dilution The optimal antigen unmasking condition is a function of temperature (90 to 100°C) and incubation time (5-30 minutes) and should be established by each investigator.

The Retreive-All working solution should be prepared by mixing 100 mL of 10x Retrieve-All into 900mL of water.

Protocol
1. Deparaffinize sections, rehydrate through graded alcohols, DI water, into PBS for 5 minutes.
2. Preheat Retrieve-All buffer to 92°C*.
Retrieve-All Antigen Unmasking System 1: Universal, 10x (SIGNET)3. Place slides into the preheated Retrieve-All buffer.
4. Incubate for 10 minutes at 92°C.
5. Remove the container with slides and let it cool at room temperature for 5-10 minutes.
6. Rinse in distilled water or PBS.
7. Proceed with usual immunohistochemical staining.

Notes *Concentrated buffers (100x) may show minimal precipitation after long term storage without any effect to the HIER.

Storage Store at room temperature.

Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval.

Retrieve-All Antigen Unmasking System 1: Universal, 10x (SIGNET)

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Description		Retrieve-All Antigen Unmasking System 1: Universal (pH 8); 10x (Formerly Signet Catalog No.: 1914)
Intended Use		Research Use Only (RUO) This product is sold for laboratory research use only, not for human or in-vivo use.
Form		Retrieve-All Antigen Unmasking System 1: Universal, 10x (SIGNET)Antigen Unmasking Solution
Specificity		Retrieve-All is an antigen un-masking solution available in the following three pH formulas for use in heat induced un-masking procedures: Retrieve-All 1 (Universal pH 8) Retrieve-All 2 (Basic pH 10) Retrieve-All 3 (Acidic pH 4.8)
Suggested Working Dilution		The optimal antigen unmasking condition is a function of temperature (90 to 100°C) and incubation time (5-30 minutes) and should be established by each investigator. The Retreive-All working solution should be prepared by mixing 100 mL of 10x Retrieve-All into 900mL of water. Protocol 1. Deparaffinize sections, rehydrate through graded alcohols, DI water, into PBS for 5 minutes. 2. Preheat Retrieve-All buffer to 92°C*. Retrieve-All Antigen Unmasking System 1: Universal, 10x (SIGNET)3. Place slides into the preheated Retrieve-All buffer. 4. Incubate for 10 minutes at 92°C. 5. Remove the container with slides and let it cool at room temperature for 5-10 minutes. 6. Rinse in distilled water or PBS. 7. Proceed with usual immunohistochemical staining.
Notes		*Concentrated buffers (100x) may show minimal precipitation after long term storage without any effect to the HIER.
Storage		Store at room temperature.
Warranty/Conditions		Covance products may not be resold or modified for resale without prior written approval.