Description | | Retrieve-All Antigen Unmasking System 1: Universal (pH 8); 1x (Formerly Signet Catalog No.: 1912) |
Intended Use | | **Research Use Only (RUO)** This product is sold for laboratory research use only, not for human or in-vivo use. |
Form | | Antigen Unmasking Solution |
Specificity | | Retrieve-All Antigen Unmasking System 1: Universal, 1x (SIGNET)Retrieve-All is an antigen un-masking solution available in the following three pH formulas for use in heat induced un-masking procedures: Retrieve-All 1 (Universal pH 8) Retrieve-All 2 (Basic pH 10) Retrieve-All 3 (Acidic pH 4.8) |
Suggested Working Dilution | | Retrieve-All Antigen Unmasking System 1: Universal, 1x (SIGNET)The optimal antigen unmasking condition is a function of temperature (90 to 100°C) and incubation time (5-30 minutes) and should be established by each investigator. Protocol 1. Deparaffinize sections, rehydrate through graded alcohols, DI water, into PBS for 5 min. 2. Preheat Retrieve-All buffer to 92°C*. 3. Place slides into the preheated Retrieve-All buffer. 4. Incubate for 10 minutes at 92°C. 5. Remove the container with slides and let it cool at room temperature for 5-10 minutes. 6. Rinse in distilled water or PBS. 7. Proceed with usual immunohistochemical staining. |
Notes | | * Concentrated buffers (100x) may show minimal precipitation after long term storage without any effect to HIER |
Storage | | Store at room temperature. |
Warranty/Conditions | | Covance products may not be resold or modified for resale without prior written approval. |