Description | | Purified Monoclonal Antibody against Vascular Endothelial Growth Factor (VEGF) (Formerly Signet Catalog Nos: 738-01, 738-16 L1 Predilute, 738-26 L2 Predilute) |
Intended Use | | **Analyte Specific Reagent** Analytical and performance characteristics are not established. |
Clone | | VG-1 |
Form | | Purified Antibody (in 0.01M PBS + 0.1% NaN3 + 1% BSA) |
Host | | Vascular Endothelial Growth Factor (VEGF) Monoclonal Antibody, Purified (SIGNET)Mouse |
Species Reactivity | | Human |
IsoType | | IgG1 |
Specificity | | Signet’s Vascular Endothelial Growth Factor (VEGF) antibody detects the 121, 165, and 185 VEGF isoforms in routinely fixed specimens. VEGF is a 19-22kDa angiogenic growth factor that promotes the formation of new blood vessels. VG-1 is useful for studies of VEGF and angiogenesis in human tissues. This antibody was generated against a recombinant VEGF protein. Concentrated Format: SIG-3738-1000 [1 mL] Prediluted Formats: SIG-3738-16 [6 mL] SIG-3738-26 [6 mL] Both are ready-to-use with either a Biotin-based (USA Ultra Streptavidin Detection, SIG-32250) or Biotin-free (AcuityAdvanced Polymer Detection, SIG-32902) detection systems. |
Uses | | Vascular Endothelial Growth Factor (VEGF) Monoclonal Antibody, Purified (SIGNET)This antibody is effective in immunohistochemistry (IHC). |
Suggested Working Dilution | | The optimal working dilution should be determined for each specific assay condition. - IHC: ≥1:40 (concentrated format) when used with Biotin based detection systems such as USA Ultra Streptavidin Detection (SIG-32250).
Tissue Sections: Formalin-fixed, paraffin-embedded tissues Pretreatment: Heat induced epitope retrieval with 0.1M EDTA. Incubation: 60 minutes at room temperature |
Notes | | Positive tissue (human): Corpus Luteum, VEGF165 recombinant protein, astrocytoma or hemoangiosarcoma |
Storage | | Store between 2-8°C. |
References | | Giatromanolaki A, et al. DEC1 (STRA13) protein expression relates to hypoxia- inducible factor 1-alpha and carbonic anhydrase-9 overexpression in non-small cell lung cancer. J Pathol 200(2):222-8, 2003. Turley H, et al. Expression of VEGF in routinely fixed material using a new monoclonal antibody VG1. J Pathol 186(3):313-318, 1998. |
Warranty/Conditions | | Covance products may not be resold or modified for resale without prior written approval. |