Description | | Purified Monoclonal Antibody against Chromogranin A (LK2H10) (Formerly Signet Catalog Nos: 215-01, 215-16 L1 Predilute, 215-26 L2 Predilute, 215-36 LV Predilute) |
Intended Use | | ** In Vitro Diagnostic (IVD) ** |
Clone | | LK2H10 |
Form | | Purified Antibody (in PBS + 0.1% NaN3 + 1% BSA) |
Host | | Mouse |
Species Reactivity | | Chromogranin A Monoclonal Antibody, Purified (SIGNET)Human |
IsoType | | IgG1/kappa |
Specificity | | The anti-Chromogranin A antibody recognizes a 68kDa protein tht is expressed by most cells of the diffuse neuroendocrine system. Clone LK2H10 has been shown to stain both normal and neoplastic endocrine tissue containing secretory granules. In tumors with abundant secretory granules, staining is intense and diffuse. In tumors with few granules (e.g. Merkel cell carcinoma), focal staining is observed. Endocrine tissues without secretory granules and non-endocrine tissues do not stain with clone LK2H10. This antibody was developed using human pheochromocytoma. Concentrated Format: SIG-3215-1000 [1 mL] Prediluted Formats: SIG-3215-16 [6 mL] is ready to use with Peroxidase Anti- Peroxidase detection systems. SIG-3215-26 [6 mL] is ready-to-use with Biotin-based detection systems such as USA Ultrastreptavidin Detection (SIG-32250). SIG-3215-36 [6 mL] is ready-to-use with Biotin based detection systems on Ventana ES and Ventana NexES automated immunostainers. |
Uses | | Chromogranin A Monoclonal Antibody, Purified (SIGNET)This antibody is effective in immunohistochemistry (IHC). |
Suggested Working Dilution | | The optimal working dilution should be determined for each specific assay condition. - IHC: ≥1:40 (concentrated format) for Biotin based detection systems such as USA Ultra Streptavidin Detection (SIG-32250)
Tissue Sections: Formalin-fixed, paraffin-embedded tissues Pretreatment: Not required Incubation: 20-60 minutes at room temperature |
Notes | | Positive tissue (human): Pancreas |