Neurofilaments, Phosphorylated (SMI 31) Monoclonal Antibody, Purified_详细资料

产品信息

靶点：来电咨询浓度： 1mg/1ml 应用范围：科研使用宿主： Goat，Rabbit，Mouse 适应物种：人，大鼠，小鼠，兔标记物：见说明书克隆性：多克隆保存条件： -20℃保存形态：液态/粉状

Neurofilaments, Phosphorylated (SMI 31) Monoclonal Antibody, Purified保存于运输说明：

Store at -20°C.

详细信息：

Description Purified form of monoclonal antibody to neurofilaments, phosphorylated epitope, Clone SMI 31

Intended Use **Research Use Only (RUO)**

This product is sold for laboratory research use only, not for human or in-vivo use.

Clone SMI-31

Form Purified Antibody (in PBS + Thimerosal)

Host Mouse

Species Reactivity Neurofilaments, Phosphorylated (SMI 31) Monoclonal Antibody, Purified Mammalian, Chicken, Xenopus

IsoType IgG1

[Ab] 1 mg/mL

Specificity SMI 31 reacts with a phosphorylated epitope in extensively phosphorylated neurofilament H and, to a lesser extent, with neurofilament M in most mammalian species, as well as in chicken and frog (Xenopus). Immunocytochemically, SMI 31 reacts broadly with thick and thin axons and some dendrites such as basket cell dendrites, but not Purkinje cell dendrites. Nerve cell bodies are generally unreactive. Other cells and tissues are unreactive except for peripheral axons. Phosphatase treatment of tissue sections or Western blots abolishes reaction with SMI 31. Staining is unaffected by trypsin. In pathological conditions, reaction with SMI 31 may be found also in neuronal cell bodies. Aberrant phosphorylation of neurofilament H in cell bodies can be demonstrated in neuronal cell cultures with SMI 31 by agents that induce stress-activated protein kinase. In its reaction with paired helical filaments in hereditary inclusion body myopathy, SMI 31 colocalizes with nitric oxide synthase, suggesting that oxidative stress may play a role in the pathogenic cascade of such degenerative diseases. SMI 31 co-immunoprecipitates neurofilament-associated kinase (NAK 115) via reaction of the antibody with the tail domain of neurofilament H.

Uses This antibody is effective in immunoblotting, immunohistochemistry (IHC), immunocytochemistry and ELISA.

Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition.

Western blot: 1:1,000

IHC: 1:1,000

ELISA: 1:1,000

The extent of permissible dilution of SMI 31 beyond those recommended for general application depends upon nature and concentration of the antigen examined, species of the antigen, method of fixation and kind of section examined.

Tissue Preparation: SMI 31 reacts on Western blots, paraffin, vibratome and frozen tissue sections as well as with neuronal cell cultures. Tissues and cultures can be fixed in a variety of paraformaldehyde or formaldehyde-containing fixatives including Bouins. "Recovery" of antigen in tissue that has been formalin-fixed for long periods of time may be accomplished by autoclaving of deparaffinized sections in distilled water (Shin et al, Lab Invest, 64:693, 1991) or by boiling sections or tissue blocks immersed in tris buffered saline, pH 9.0, in a microwave oven for 15 min (Evers and Uylings, J Neurosci Methods 72:197, 1997). Post-fixation in cold methanol or methanol/hydrogen peroxide greatly facilitates access of SMI 31 in frozen sections or thick sections of tissue perfused with 4% paraformaldehyde and in tissue cultures. These antibodies can be used for neurofilament detection in trypsin-treated tissue.

Storage Neurofilaments, Phosphorylated (SMI 31) Monoclonal Antibody, Purified Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.

References Raina AK, Takeda A, Nunomura A, Perry G, Smith MA. Genetic evidence for oxidative stress in Alzheimers disease. Neuroreport 10:1, 1999.

Yang CC, Alvarez RR, Engel WK, Heller SL, Askansas. Nitric oxide-induced oxidative stress in autosomal recessive and dominant inclusion-body myopathies. Brain 121:1089, 1998.

Giasson BI, Mushynski WE. Aberrant stress-induced phosphorylation of perikaryal neurofilaments. J Biol Chem 271:30404, 1996.

Mirabella M, Alvarez RB, Bilak M, Engel WK, Askansas V. Differences in expression of phosphorylated tau epitopes between sporadic inclusion-body myositis and hereditary inclusion-body myopathies. J Neurpoath Exp Neurol 55:774, 1996.

Xiao J, Monteiro MJ. Identification and characterization of a novel (115 kDa) neurofilament-associated kinase. J Neurosci 14:1820, 1994.

Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval.

Neurofilaments, Phosphorylated (SMI 31) Monoclonal Antibody, Purified

xy-0583xy   ABL1(v-abl Abelson murine leukemia viral oncogene homolog 1)ABL1抗原
xy-2940xy   FUCA1/Alpha L fucosidase I peptideα-L岩藻糖苷酶抗原
xy-0275xy   Bassoon(BSN)
xy-0127xy   Bax peptideBax（多肽抗原）
xy-0409xy-FITxy   Leptin peptide/FITC荧光素FITC标记瘦素抗原
xy-0032xy   Bcl-2(抗原)Bcl-2（多肽抗原）
xy-0248xy   BDNF (Brain-derived Neurotrophin factor)脑源神经营养因子(脑衍化神经营养因子)（多肽片断抗原）
xy-2249xy   ACA11 peptide拟南芥ACA11蛋白多肽抗原
xy-2247xy   AHA3 peptide植物蛋白AHA3抗原（拟南芥）
xy-0217xy   bFGF碱性成纤维细胞生长因子（多肽抗原）
xy-1004xy   ACE2(Angiotensin converting enzyme 2)血管紧张素转换酶2抗原
xy-0044xy   BNP脑钠素（多肽抗原）
xyb-0044xy   BNP/Biotin生物素化脑钠素多肽
xy-0324xy   C5过敏毒素C5（补体C5）抗原
xy-0091xy   CA125卵巢癌抗原
xy-0043xy   CAD(Caspase-Activated Deoxyribonuclease)Caspase 激活的脱氧核糖核酸酶（抗原）
xy-3550xy   MYOG肌红蛋白抗原
xy-0095xy   Calponin 1钙调节蛋白-1（抗原）
xy-3679xy   COMP软骨寡聚基质蛋白抗原
xy-0169xy   Caspase-1 (ICE; CASP-1；P45)天冬氨酸-胱氨酸特异性蛋白酶家族（抗原）
xy-0081xy   Caspase-3 (CPP32)半胱胺酸蛋白酶蛋白-3（抗原）
xy-0151xy   Caspase-6 (CT)半胱胺酸蛋白酶蛋白-6（抗原）
xy-0049xy   Caspase-9白介素1-β转化酶样凋亡蛋白酶6（抗原）
xy-0050xy   Caspase-9 白介素1-β转化酶样凋亡蛋白酶6（抗原）
xy-0092xy   Caspase-10 半胱胺酸蛋白酶-10（抗原）
xy-0053xy   Caspase-13 半胱胺酸蛋白酶蛋白-13（抗原）
xy-0079xy   CD19(B-lymphocyte antigen CD19)CD19
xy-2167xy   DRADA/ADAR1(Double-stranded RNA-specific adenosine deaminase)N terminal双链RNA腺苷酸脱氨基酶抗原（N端）
xy-2168xy   DRADA/ADAR1(Double-stranded RNA-specific adenosine deaminase)双链RNA腺苷酸脱氨基酶抗原
xy-4593xy   MMP9基质金属蛋白酶9
xy-4622xy   Mycobacterium bovis 牛结核杆菌菌体蛋白
xy-4687xy   AMH/MISMuellerianYZ因子抗原
xy-2231xy   AEG-1 peptideAEG-1抗原
xy-0195xy   CD31 (PECAM-1)血小板内皮细胞黏附分子-1（抗原）
xy-1164xy   ADFP/ADRP/adipophilin 脂肪组织分化相关蛋白抗原
xy-0298xy   Immunoglobuin binding protein-1(CD79A)IGBP-1免疫球蛋白结合蛋白-1

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Description		Purified form of monoclonal antibody to neurofilaments, phosphorylated epitope, Clone SMI 31
Intended Use		Research Use Only (RUO) This product is sold for laboratory research use only, not for human or in-vivo use.
Clone		SMI-31
Form		Purified Antibody (in PBS + Thimerosal)
Host		Mouse
Species Reactivity		Neurofilaments, Phosphorylated (SMI 31) Monoclonal Antibody, Purified Mammalian, Chicken, Xenopus
IsoType		IgG1
[Ab]		1 mg/mL
Specificity		SMI 31 reacts with a phosphorylated epitope in extensively phosphorylated neurofilament H and, to a lesser extent, with neurofilament M in most mammalian species, as well as in chicken and frog (Xenopus). Immunocytochemically, SMI 31 reacts broadly with thick and thin axons and some dendrites such as basket cell dendrites, but not Purkinje cell dendrites. Nerve cell bodies are generally unreactive. Other cells and tissues are unreactive except for peripheral axons. Phosphatase treatment of tissue sections or Western blots abolishes reaction with SMI 31. Staining is unaffected by trypsin. In pathological conditions, reaction with SMI 31 may be found also in neuronal cell bodies. Aberrant phosphorylation of neurofilament H in cell bodies can be demonstrated in neuronal cell cultures with SMI 31 by agents that induce stress-activated protein kinase. In its reaction with paired helical filaments in hereditary inclusion body myopathy, SMI 31 colocalizes with nitric oxide synthase, suggesting that oxidative stress may play a role in the pathogenic cascade of such degenerative diseases. SMI 31 co-immunoprecipitates neurofilament-associated kinase (NAK 115) via reaction of the antibody with the tail domain of neurofilament H.
Uses		This antibody is effective in immunoblotting, immunohistochemistry (IHC), immunocytochemistry and ELISA.
Suggested Working Dilution		The optimal working dilution should be determined for each specific assay condition. Western blot: 1:1,000 IHC: 1:1,000 ELISA: 1:1,000 The extent of permissible dilution of SMI 31 beyond those recommended for general application depends upon nature and concentration of the antigen examined, species of the antigen, method of fixation and kind of section examined. Tissue Preparation: SMI 31 reacts on Western blots, paraffin, vibratome and frozen tissue sections as well as with neuronal cell cultures. Tissues and cultures can be fixed in a variety of paraformaldehyde or formaldehyde-containing fixatives including Bouins. "Recovery" of antigen in tissue that has been formalin-fixed for long periods of time may be accomplished by autoclaving of deparaffinized sections in distilled water (Shin et al, Lab Invest, 64:693, 1991) or by boiling sections or tissue blocks immersed in tris buffered saline, pH 9.0, in a microwave oven for 15 min (Evers and Uylings, J Neurosci Methods 72:197, 1997). Post-fixation in cold methanol or methanol/hydrogen peroxide greatly facilitates access of SMI 31 in frozen sections or thick sections of tissue perfused with 4% paraformaldehyde and in tissue cultures. These antibodies can be used for neurofilament detection in trypsin-treated tissue.
Storage		Neurofilaments, Phosphorylated (SMI 31) Monoclonal Antibody, Purified Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
References		Raina AK, Takeda A, Nunomura A, Perry G, Smith MA. Genetic evidence for oxidative stress in Alzheimers disease. Neuroreport 10:1, 1999. Yang CC, Alvarez RR, Engel WK, Heller SL, Askansas. Nitric oxide-induced oxidative stress in autosomal recessive and dominant inclusion-body myopathies. Brain 121:1089, 1998. Giasson BI, Mushynski WE. Aberrant stress-induced phosphorylation of perikaryal neurofilaments. J Biol Chem 271:30404, 1996. Mirabella M, Alvarez RB, Bilak M, Engel WK, Askansas V. Differences in expression of phosphorylated tau epitopes between sporadic inclusion-body myositis and hereditary inclusion-body myopathies. J Neurpoath Exp Neurol 55:774, 1996. Xiao J, Monteiro MJ. Identification and characterization of a novel (115 kDa) neurofilament-associated kinase. J Neurosci 14:1820, 1994.
Warranty/Conditions		Covance products may not be resold or modified for resale without prior written approval.