Loricrin Polyclonal Antibody, Purified_详细资料

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靶点：来电咨询浓度： 1mg/1ml 应用范围：科研使用宿主： Goat，Rabbit，Mouse 适应物种：人，大鼠，小鼠，兔标记物：见说明书克隆性：多克隆保存条件： -20℃保存形态：液态/粉状

Loricrin Polyclonal Antibody, Purified保存于运输说明：

详细信息：

Description Polyclonal Antibody against Mouse Loricrin (AF 62)

Intended Use ** In Vitro Diagnostic (IVD) **

Form Affinity Purified Antibody (Stored in PBS+ 0.03% Thimerosal)

Host Rabbit

Species Reactivity Human, Mouse

[Ab] 1 mg/mL

Specificity Loricrin Polyclonal Antibody, Purified This monospecific polyclonal antibody was raised against a peptide sequence derived from the C-terminus of the mouse loricrin protein. It was subsequently purified using peptide affinity chromatography.

Uses This antibody is effective in immunoblotting (WB), immunofluorescence (IF) and immunohistochemistry (IHC). This antibody has been used successfully in both frozen and paraffin embedded tissues.

Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition.

WB: 1:1,000*

IF: 1:500

IHC: 1:1,000 with either biotin based detection systems such as USA Ultra Streptavidin Detection (SIG-32250) or polymer based detection systems such as ACUITYAdvanced Biotin Free Polymer Detection (SIG-32902).
Tissue Sections: Paraffin-embedded tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Citrate Buffer Retrieval solution (SIG-31932, SIG-31934).
Incubation: 60 minutes at room temperature

Notes Predicted MW = In mouse, expect a 57 kDa band while in human, the band may fall between 26-30 kDa.

Storage When stored at ≤20°C, product is stable until the date shown on the label. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. If thawed and stored at 2-8°C, product is stable for 24 months from the date of thaw or until the expiry date on the label, whichever comes first.

References Loricrin Polyclonal Antibody, Purified Hu Y, Baud V, Oga T, Kim KI, Yoshida K, Karin M. IKKalpha controls formation of the epidermis independently of NF-kappaB. Nature 410:710-714, 2001.

Yuspa SH, Kilkenny AD, Steinert PM, Roop DR. Expression of murine epidermal differentiation markers is tightly regulated by restricted extracellular calcium concentrations in-vitro. J Cell Biol 109:1207-1217, 1989. [General]

Roop DR, Cheng CK, Titterington L, Meyers CA, Stanley JR, Steinert PM, Yuspa SH. Synthetic peptides corresponding to keratin subunits elicit highly specific antibodies. J Biol Chem 259:8037-8040, 1984. [General]

Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval.

Loricrin Polyclonal Antibody, Purified

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xy-0309R-xyy5   Rabbit Anti-pig IgG/Cy5Cy5标记的兔抗猪IgG
xy-0309R-xyy5.5   Rabbit Anti-pig IgG/Cy5.5Cy5.5标记的兔抗猪IgG
xy-0309R-xyy7   Rabbit Anti-pig IgG/Cy7Cy7标记的兔抗猪IgG
xy-0309R-AF555   Rabbit Anti-pig IgG/Alexa Fluor 555Alexa Fluor 555标记的兔抗猪IgG
xy-0309R-AF647   Rabbit Anti-pig IgG/Alexa Fluor 647Alexa Fluor 647标记的兔抗猪IgG
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xy-0309R-FITxy   Rabbit Anti-pig IgG/FITCFITC标记的兔抗猪IgG
xy-0309R-RBITxy   Rabbit Anti-pig IgG/RBITC罗丹明标记的兔抗猪IgG
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xy-0403R-xyy7   Rabbit anti-MG IgG/Cy7Cy7标记的兔抗长爪沙鼠IgG
xy-0403R-RBITxy   Rabbit anti-MG IgG/RBITC罗丹明标记的兔抗长爪沙鼠IgG
xy-0403R-FITxy   Rabbit anti-MG IgG/FITCFITC标记的兔抗长爪沙鼠IgG
xy-0403R-Axy   Rabbit anti-MG IgG/AP碱性磷酸酶（AP）标记的兔抗长爪沙鼠IgG
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Loricrin Polyclonal Antibody, Purified

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Description		Polyclonal Antibody against Mouse Loricrin (AF 62)
Intended Use		In Vitro Diagnostic (IVD)
Form		Affinity Purified Antibody (Stored in PBS+ 0.03% Thimerosal)
Host		Rabbit
Species Reactivity		Human, Mouse
[Ab]		1 mg/mL
Specificity		Loricrin Polyclonal Antibody, Purified This monospecific polyclonal antibody was raised against a peptide sequence derived from the C-terminus of the mouse loricrin protein. It was subsequently purified using peptide affinity chromatography.
Uses		This antibody is effective in immunoblotting (WB), immunofluorescence (IF) and immunohistochemistry (IHC). This antibody has been used successfully in both frozen and paraffin embedded tissues.
Suggested Working Dilution		The optimal working dilution should be determined for each specific assay condition. WB: 1:1,000* IF: 1:500 IHC: 1:1,000 with either biotin based detection systems such as USA Ultra Streptavidin Detection (SIG-32250) or polymer based detection systems such as ACUITYAdvanced Biotin Free Polymer Detection (SIG-32902). Tissue Sections: Paraffin-embedded tissues Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Citrate Buffer Retrieval solution (SIG-31932, SIG-31934). Incubation: 60 minutes at room temperature
Notes		Predicted MW = In mouse, expect a 57 kDa band while in human, the band may fall between 26-30 kDa.
Storage		When stored at ≤20°C, product is stable until the date shown on the label. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. If thawed and stored at 2-8°C, product is stable for 24 months from the date of thaw or until the expiry date on the label, whichever comes first.
References		Loricrin Polyclonal Antibody, Purified Hu Y, Baud V, Oga T, Kim KI, Yoshida K, Karin M. IKKalpha controls formation of the epidermis independently of NF-kappaB. Nature 410:710-714, 2001. Yuspa SH, Kilkenny AD, Steinert PM, Roop DR. Expression of murine epidermal differentiation markers is tightly regulated by restricted extracellular calcium concentrations in-vitro. J Cell Biol 109:1207-1217, 1989. [General] Roop DR, Cheng CK, Titterington L, Meyers CA, Stanley JR, Steinert PM, Yuspa SH. Synthetic peptides corresponding to keratin subunits elicit highly specific antibodies. J Biol Chem 259:8037-8040, 1984. [General]
Warranty/Conditions		Covance products may not be resold or modified for resale without prior written approval.