Fibrillarin Monoclonal Antibody_详细资料

产品信息

靶点：来电咨询浓度： 1mg/1ml 应用范围：科研使用宿主： Goat，Rabbit，Mouse 适应物种：人，大鼠，小鼠，兔标记物：见说明书克隆性：多克隆保存条件： -20℃保存形态：液态/粉状

Fibrillarin Monoclonal Antibody详细信息：

Description Monoclonal antibody against Fibrillarinn/Nop1p

Clone 38F3

Form Sterile-Filtered Cell Culture Supernatant (contains 10mM sodium azide)

Host Mouse

Species Reactivity Human, Rat, Drosophila, S. pombe, C. elegans, and plants

IsoType IgG1

Specificity Fibrillarin Monoclonal Antibody Nop1p was originally identified as a nucleolar protein of bakers yeast, S. cerevisiae (accession P15646). The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli. It is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein apparently found in all eukaryotes and archea generally called fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. This means that suitably cross-reactive antibodies to Nop1p/fibrillarin, like MCA-38F3, can be used to reveal nucleoli and study fibrillarin/Nop1p in all eukaryotes and archea tested to date.

38F3 was raised against yeast nuclear preparations. The subsequent hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates. This clone stains a single ~34kDa band on western blotting and shows a clear and strong punctate staining of yeast nuclei.

Uses This antibody is useful for immunoblotting (WB), immunofluorescence (IF) and immunocytochemistry.

Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval.

WB: 1:500 (mammalian lysates), 1:2,000 (yeast cell lysates), 1:10,000 (yeast nuclear fraction)*

IF: 1:500 (mammalian), 1:1,000-5,000 (yeast)

Notes Fibrillarin Monoclonal Antibody Predicted MW = 34kD

Storage Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

References Newton K, Petfalski E, Tollervey D, Caceres JF. Fibrillarin is essential for early development and required for accumulation of an intron-encoded small nucleolar RNA in the mouse. Mol. Cell Biol. 23:8519-8527, 2003.

Ochs RL, Lischwe MA, Spohn WH, Busch H. Fibrillarin: a new protein of the nucleolus identified by autoimmune sera. Biol. Cell 54:123-133, 1985.

Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval.

Fibrillarin Monoclonal Antibody

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Description		Monoclonal antibody against Fibrillarinn/Nop1p
Clone		38F3
Form		Sterile-Filtered Cell Culture Supernatant (contains 10mM sodium azide)
Host		Mouse
Species Reactivity		Human, Rat, Drosophila, S. pombe, C. elegans, and plants
IsoType		IgG1
Specificity		Fibrillarin Monoclonal Antibody Nop1p was originally identified as a nucleolar protein of bakers yeast, S. cerevisiae (accession P15646). The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli. It is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein apparently found in all eukaryotes and archea generally called fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. This means that suitably cross-reactive antibodies to Nop1p/fibrillarin, like MCA-38F3, can be used to reveal nucleoli and study fibrillarin/Nop1p in all eukaryotes and archea tested to date. 38F3 was raised against yeast nuclear preparations. The subsequent hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates. This clone stains a single ~34kDa band on western blotting and shows a clear and strong punctate staining of yeast nuclei.
Uses		This antibody is useful for immunoblotting (WB), immunofluorescence (IF) and immunocytochemistry.
Suggested Working Dilution		The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval. WB: 1:500 (mammalian lysates), 1:2,000 (yeast cell lysates), 1:10,000 (yeast nuclear fraction)* IF: 1:500 (mammalian), 1:1,000-5,000 (yeast)
Notes		Fibrillarin Monoclonal Antibody Predicted MW = 34kD
Storage		Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
References		Newton K, Petfalski E, Tollervey D, Caceres JF. Fibrillarin is essential for early development and required for accumulation of an intron-encoded small nucleolar RNA in the mouse. Mol. Cell Biol. 23:8519-8527, 2003. Ochs RL, Lischwe MA, Spohn WH, Busch H. Fibrillarin: a new protein of the nucleolus identified by autoimmune sera. Biol. Cell 54:123-133, 1985.
Warranty/Conditions		Covance products may not be resold or modified for resale without prior written approval.