Stemgent® Affinity Purified anti-Human TRA-1-81 Antibody

Stemgent® Affinity Purified anti-Human TRA-1-81 Antibody详细信息：
Product Overview

 

The TRA-1-81 monoclonal antibody reacts with a pluripotent-stem-cellspecific antigen expressed upon the surface of human embryonic stem (ES) cells, embryonal carcinoma (EC) cells, and embryonic germ (EG) cells. The expression of TRA-1-81 antigen is stage-specific and can be used to characterize embryonic cells and monitor their differentiation. As human EC and ES cells undergo differentiation, expression of TRA-1-81 antigen is lost. TRA-1-81 antibody recognizes a carbohydrate epitope expressed on podocalyxin, a sialoglycoprotein structurally related to CD34. Podocalyxin is a transmembrane glycoprotein which has been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.

Stemgent® Affinity Purified anti-Human TRA-1-81 AntibodyProduct Specifications

Imunogen    Human embryonal carcinoma cell line 2102Ep
Reactivity    Human
Clone    TRA-1-81
Isotype    Mouse IgM, κ
Formulation    Phosphate-buffered solution, pH 7.2, containing ≤0.09% sodium azide.
Preparation    The antibody was purified by affinity chromatography.
Storage    Store undiluted at 4°C. Do not freeze.
Stability    Stable for 6 months from date of receipt when stored as directed.
Quality Control    Tested by flow cytometry and immunocytochemistry to ensure product quality.
Recommended Usage    0.25 μg per 1x106 viable cells for flow cytometry and 5 μg/ml for immunocytochemistry.
Stemgent® Affinity Purified anti-Human TRA-1-81 AntibodyTechnical Documents

Specifications

09-0011 Specifications Sheet
MSDS

Material Safety Data Sheet
Protocols

General Protocol for Immunocytochemistry
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Stemgent® Affinity Purified anti-Human TRA-1-81 Antibody
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