Stemgent® Affinity Purified anti-Mouse/Human Rex1 Antibody

Stemgent® Affinity Purified anti-Mouse/Human Rex1 Antibody详细信息：
Product Overview

 

Affinity Purified anti-Mouse/Human Rex1 was screened on human and mouse ES cells using ICC and flow cytometry and selected as the best Rex1 antibody available for researchers needing to demonstrate pluripotency. Rex 1, also termed zinc-finger protein-42 (Zfp42), is a member of the YY1 sub-family of transcription factors1, and has emerged as an important stem cell marker in both mouse and human undifferentiated embryonic stem (ES) cells2. Expression of Rex1 is regulated by Nanog, Oct4, and Sox2 at the level of transcription1-3.

 

Stemgent® Affinity Purified anti-Mouse/Human Rex1 AntibodyReferences

Melissa B Rogers, Betsy A. Hosler, Lorraine J. Gudas. (1999) Specific Expression of a Retinoic Acid-Regulated, Zinc-Finger Gene, Rex-1, in Preimplantation Embryos, Trophoblast and Spermatocytes. Development 113:815-824.
Brivanlou AH, Gage FH, Jaenisch R, Jessell T, Melton D, Rossant J. (2003) Setting Standards for Human Embryonic Stem Cells. Science 300:913–916.
Wenjing Shi, Hui Wang, Guangjin Pan, Yijie Geng, Yunqian Guo, Duanqing Pei. (2006) Regulation of the Pluripotency Marker Rex-1 by Nanog and Sox2. The Journal of Biological Chemistry 281(33):23319–23325.
Product Specifications

Immunogen    Synthetic mouse Rex1 peptide (within residues 1-100) conjugated to KLH
Reactivity    Mouse and Human
Stemgent® Affinity Purified anti-Mouse/Human Rex1 AntibodyClone    Polyclonal
Isotype    Rabbit IgG
Formulation    Phosphate-buffered solution pH 7.2 containing <0.1% sodium azide and <1.0% BSA.
Preparation    The antibody was purified by immunogen affinity chromatography.
Storage    Store undiluted at 4oC. Do not freeze.
Stability    Stable for 6 months from date of receipt when stored as directed.
Quality Control    Tested by immunocytochemistry (Figure 1) and flow cytometry (Figure 2).
Recommended Usage    Dilute 1:100 for immunocytochemistry applications and 1:5 for flow cytometry applications.
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Stemgent® Affinity Purified anti-Mouse/Human Rex1 Antibody
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