Stemgent® Affinity Purified anti-Mouse/Human SSEA-1 Antibody

Stemgent® Affinity Purified anti-Mouse/Human SSEA-1 Antibody详细信息：
Product Overview

SSEA-1 is an antigenic epitope defined as a Lewis X carbohydrate structure. It is expressed on murine embryonal carcinoma cells, murine embryonic stem cells, and murine and human germ cells.

References

Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., and Yamanaka, S. (2007). Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors. Cell 131, 861-872.
Takahashi, K. and Yamanaka, S. (2006). Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663-676.
Solter, D. and Knowles, B.B. (1978). Monoclonal antibody defining a stagespecific mouse embryonic antigen (SSEA-1). Proc. Natl. Acad. Sci. USA 75, 5565-5569.
Gooi H.C., Feizi T., Kapadia A., Knowles B.B., Solter D., Evans M.J. (1981). Stage-specific embryonic antigen involves alpha 1 goes to 3 fucosylated type 2 blood group chains. Nature 292, 156-158.
Product Specifications

Stemgent® Affinity Purified anti-Mouse/Human SSEA-1 AntibodyImunogen    Mouse F9 Teratocarcinoma Stem Cells (X-irradiated)
Reactivity    Mouse
Cross-Reactivity    Human
Clone    MC-480
Isotype    Mouse IgM, κ
Size    50 μg (0.1 ml at 0.5 mg/ml)
Formulation    Phosphate-buffered solution, pH 7.2, containing ≤0.09% sodium azide.
Preparation    The antibody was purified by affinity chromatography.
Storage    Store undiluted at 4°C. Do not freeze.
Stability    Stable for 6 months from date of receipt when stored as directed.
Quality Control    Tested by flow cytometry and immunocytochemistry to ensure product quality.
Recommended Usage    0.25 μg per 1x106 viable cells for flow cytometry and 5 μg/ml for immunocytochemistry.
General Protocol for Immunofluorescent Staining Using Flow Cytometry    
Isolate and dissociate cells to a single cell suspension. Collect cells in a 15 ml conical tube.
Centrifuge the cell suspension at 300 x g for 5 minutes at 4°C. Resuspend the cell pellet to a concentration of 0.2x107 to 1x107 cells per ml in PBS. Keep cells on ice.
Stemgent® Affinity Purified anti-Mouse/Human SSEA-1 AntibodyFor each sample, add 100 μl of the cell suspension to a 4 ml roundbottom tube and add the appropriate amount (10-20 μl) of the purified antibody.
Incubate at 4°C for 30 minutes to 1 hour.
Wash the cells by adding PBS to bring cell suspension to 4 ml. Centrifuge at 300 x g for 5 minutes at 4°C.
Aspirate and agitate the cell pellet. Then add the appropriate amount of FITC-conjugated anti-mouse IgM antibody to each sample.
Incubate at 4°C for 30 minutes to 1 hour.
Add PBS to bring cell suspension to 4 ml. Centrifuge at 300 x g for 5 minutes at 4°C.
Aspirate and agitate the cell pellet. Add 10 &muL (0.25 &mug) of 7- Aminoactinomycin D (cell viability dye) to each sample and incubate for 20 minutes at 4°C.
Add appropriate volume of PBS to each tube. The cells are ready to be analyzed by flow cytometry.
General Protocol for Immunocytochemistry    
Seed appropriate number of ES cells in a 12-well plate on mouse embryonic fibroblast (MEF) feeder cells. Culture cells in a 37°C incubator with 5% CO2 for 3-4 days.
Wash the cells gently with PBS 3 times.
Fix the cells with 500 μl fixative solution for 20 minutes at room temperature.
Wash the cells with PBS 3 times.
Block non-specific binding with 500 μl appropriate blocking buffer for 1 hour at room temperature.
Incubate the cells with 200 μl of the antibody overnight at 4°C.
Wash the cells with PBS 3 times.
Incubate with 200 μl of appropriate conjugated secondary antibody for 2 hours at room temperature. Avoid exposing the plate to light.
Wash cells with PBS 3 times.
During the last wash, add DAPI stain to a final concentration at 1 μg/ml for 5 minutes.
After the final wash with PBS, the cells are ready to be analyzed using a fluorescent microscope.
Notice to Purchaser    Purchaser represents and warrants that it will use Phycoerythrin (PE) antihuman SSEA-4 purely for research purposes, not for diagnostic use, not for resale, and not for use in humans or veterinary applications. Stemgent will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Stemgent® Affinity Purified anti-Mouse/Human SSEA-1 Antibody
xy-E11085    胱天蛋白酶9(Casp-9)ELISA试剂盒    检测范围
xy-E11086    胱天蛋白酶3(Casp-3)ELISA试剂盒    检测范围
xy-E11087    中性粒细胞弹性蛋白酶(NE)ELISA试剂盒    检测范围
xy-E11088    碳酸酐酶2(CA-2)ELISA试剂盒    检测范围
xy-E11089    解整合素样金属蛋白酶8(ADAM8)ELISA试剂盒    检测范围
xy-E11090    髓过氧化物酶(MPO)ELISA试剂盒    检测范围
xy-E11091    颗粒酶B(Gzms-B)ELISA试剂盒    检测范围
xy-E11092    颗粒酶A(Gzms-A)ELISA试剂盒    检测范围
xy-E11093    牛小肠碱性磷酸酶(CIAP)ELISA试剂盒    检测范围
xy-E11094    超氧化物歧化酶(SOD)ELISA试剂盒    检测范围
xy-E11095    二肽基肽酶Ⅳ(DPPⅣ)ELISA试剂盒    检测范围
xy-E11096    组织蛋白去乙酰化酶(HD)ELISA试剂盒    检测范围
xy-E11097    甲基化酶(Methylase)ELISA试剂盒    检测范围
xy-E11098    抗酒石酸酸性磷酸酶5b(TRACP-5b)ELISA试剂盒    检测范围
xy-E11099    磷酸肌醇3激酶(PI3K)ELISA试剂盒    检测范围
xy-E11100    蛋白激酶B(PKB)ELISA试剂盒    检测范围
xy-E11101    血红素氧合酶2(HO-2)ELISA试剂盒    检测范围
xy-E11102    丙酮酸激酶M2型同工酶(M2-PK)ELISA试剂盒    检测范围
xy-E11103    甲状腺过氧化物酶(TPO)ELISA试剂盒    检测范围
xy-E11104    αL岩藻糖苷酶(AFU)ELISA试剂盒    检测范围
xy-E11105    内皮型一氧化氮合成酶(eNOS)ELISA试剂盒    检测范围
xy-E11106    血红素氧合酶1(HO-1)ELISA试剂盒    检测范围
xy-E11107    诱导型一氧化氮合成酶(iNOS)ELISA试剂盒    检测范围
xy-E11108    磷脂酶A2(PL-A2)ELISA试剂盒    检测范围
xy-E11109    一氧化氮合成酶(NOS)ELISA试剂盒    检测范围
xy-E11110    端粒酶(TE)ELISA试剂盒    检测范围
xy-E11111    基质金属蛋白酶5(MMP-5)ELISA试剂盒    检测范围
xy-E11112    基质金属蛋白酶4(MMP-4)ELISA试剂盒    检测范围
xy-E11113    基质金属蛋白酶9/明胶酶B(MMP-9/Gelatinase B)ELISA试剂盒    检测范围
xy-E11114    肌酸激酶同工酶MB(CK-MB)ELISA试剂盒    检测范围
xy-E11115    前列腺酸性磷酸酶(PAP)ELISA试剂盒    检测范围
xy-E11116    前列腺素D合成酶(PGDS)ELISA试剂盒    检测范围
xy-E11117    胃蛋白酶原C(PG-C)ELISA试剂盒    检测范围
xy-E11118    胃蛋白酶原A(PG-A)ELISA试剂盒    检测范围
xy-E11119    尿激酶型纤溶酶原激活物受体(PLAUR/uPAR)ELISA试剂盒    检测范围
xy-E11120    尿激酶型纤溶酶原激活物(uPA)ELISA试剂盒    检测范围
xy-E11121    血管生成素受体/含免疫球蛋白样环和上皮生长因子样域酪氨酸激酶2(Tie-2)ELISA试剂盒    检测范围
xy-E11122    基质金属蛋白酶8/中性粒细胞胶原酶(MMP-8)ELISA试剂盒    检测范围
xy-E11123    基质金属蛋白酶7(MMP-7)ELISA试剂盒    检测范围
xy-E11124    基质金属蛋白酶3(MMP-3)ELISA试剂盒    检测范围
xy-E11125    基质金属蛋白酶2/明胶酶A(MMP-2/Gelatinase A)ELISA试剂盒    检测范围
xy-E11126    基质金属蛋白酶13(MMP-13)ELISA试剂盒    检测范围