Stemgent® Phycoerythrin (PE) Mouse IgG3, κ Isotype Control Antibody

Stemgent® Phycoerythrin (PE) Mouse IgG3, κ Isotype Control Antibody详细信息：
Product Overview

The mouse IgG3, κ, was chosen as an isotype control immunoglobulin after screening with a variety of resting, activated, live, and fixed mouse, rat, and human tissues.

Product Specifications

Imunogen    Trinitrophenol + KLH
Clone    MG3-35
Stemgent® Phycoerythrin (PE) Mouse IgG3, κ Isotype Control AntibodyIsotype    Mouse IgG3, κ
Formulation    1 ml at 0.025 mg/ml concentration. Supplied in a phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA.
Preparation    The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and free antibody.
Storage    Store at 4°C. Do not freeze. Light sensitive.
Stability    Stable for 6 months from date of receipt when stored as directed.
Applications    Tested by flow cytometry to ensure product quality. This antibody is used as an isotype control in immunofluorescence staining (surface or intracellular) for flow cytometric analysis.
Recommended Usage    This isotype control is used at the same concentration as the relevant antibody, typically 1 μg per 1x106 cells.
Stemgent® Phycoerythrin (PE) Mouse IgG3, κ Isotype Control AntibodyGeneral Protocol for Immunofluorescent Staining    
Isolate and dissociate cells to single cell suspension. Collect cells in a 15 ml conical tube.
Centrifuge the cell suspension at 300 x g for 5 minutes at 4°C. Re-suspend the cell pellet to a concentration of 0.2x107 to 1x107 cells per ml in PBS. Keep cells on ice.
For each sample, add 100 μl of the cell suspension to a 4 ml round-bottom tube and add the appropriate amount (5-20 μl per 1x106 viable cells) of the PE-conjugated antibody.
Incubate at 4°C for 30 minutes to 1 hour.
Add PBS to bring cell suspension to 4 ml. Centrifuge the cell suspension at 300 x g for 5 minutes at 4°C.
Aspirate and agitate the cell pellet. Add 10 μL (0.25 μg) of 7- Aminoactinomycin D (cell viability dye) to each sample and incubate for 20 minutes at 4°C.
Add appropriate volume of PBS to each tube. The cells are ready to be analyzed by flow cytometry.
Notice to Purchaser    Purchaser represents and warrants that it will use Phycoerythrin (PE) antihuman SSEA-4 purely for research purposes, not for diagnostic use, not for resale, and not for use in humans or veterinary applications. Stemgent will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Stemgent® Phycoerythrin (PE) Mouse IgG3, κ Isotype Control Antibody
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