Stemgent® Purified Rabbit anti-Mouse/Human Oct4 Antibody

Stemgent® Purified Rabbit anti-Mouse/Human Oct4 Antibody详细信息：
Product Overview

 
Octamer-4 (Oct4), a homeodomain transcription factor of the POU family (POU5F1) is involved in regulation of pluripotency during normal development. The expression of Oct4 is associated with an undifferentiated phenotype in embryonic stem (ES) cells, while gene knockdown of Oct4 promotes differentiation. As such, it is frequently used as a marker for undifferentiated ES cells or iPS (induced pluripotent stem) cells. Oct4 is one of the four key transcription factors (Oct4, Sox2, c-Myc, and Klf4) used to reprogram mouse and human fibroblasts to a pluripotent state.1-5

 
Stemgent® Purified Rabbit anti-Mouse/Human Oct4 AntibodyProduct Specifications

Immunogen    Synthetic human Oct4 peptide (within residues 300 to the C-terminus) conjugated to KLH
Reactivity    Mouse and Human
Clone    Polyclonal
Isotype    Rabbit IgG
Size    100 μl
Formulation    Phosphate-buffered solution pH 7.2 containing ≤0.09% sodium azide.
Preparation    The antibody was purified by immunogen affinity chromatography.
Storage    Store undiluted at 4°C. Do not freeze.
Stability    Stable for 6 months from date of receipt when stored as directed.
Quality Control    Tested by immunocytochemistry and flow cytometry.
Recommended Usage    Dilute 1:100 for immunocytochemistry applications and 1:5 for flow cytometry applications.
Technical Documents

Stemgent® Purified Rabbit anti-Mouse/Human Oct4 AntibodySpecifications

09-0023 Specifications Sheet
MSDS

Material Safety Data Sheet
Protocols

General Protocol for Immunocytochemistry
General Protocol for Immunofluorescent Staining of Intracellular Biomarkers Using Flow Cytometry
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