Stemgent® Purified Rabbit anti-Mouse/Human Pax6 Antibody

Stemgent® Purified Rabbit anti-Mouse/Human Pax6 Antibody详细信息：
Product Overview

The Stemgent® Purified Rabbit anti-Mouse/Human Pax-6 Antibody was screened on neural progenitors derived via directed differentiation of mouse and human ES cells. It was selected as the best Pax-6 antibody for immunocytochemistry applications. This antibody was generated against the C-terminus of the mouse Pax-6 protein, which is highly conserved among Pax-6 of various species. The protein has a predicted MW of 47-50 kDa. Pax-6 is a nuclear transcription factor essential for the development of the central nervous system, eyes, nose, and endocrine glands. The family of Pax genes encode transcriptional regulators which are major controllers of development in both vertebrates and invertebrates. The PAX protein contains two DNA-binding domains: the paired domain and the paired-type homeodomain. It is expressed in the brain and developing nervous system, and eyes. Mutations in this gene are known to cause aniridia as well as Peters anomaly, both ocular diseases. Pax-6 is known to be required for the differentiation of pancreatic islet alpha cells, and binds to the glucagon, insulin and somatostatin promoters1-6.

Stemgent® Purified Rabbit anti-Mouse/Human Pax6 AntibodyProduct Specifications

Immunogen    Peptide (QVPGSEPDMSQYWPRLQ) from the C-terminus of mouse Pax-6 protein
Reactivity    Mouse, Human
Clone    Polyclonal
Isotype    Rabbit IgG
Formulation    Phosphate-buffered solution and 0.03% thimerosal.
Preparation    This antibody was purified by affinity chromatography.
Storage    Store undiluted at -20°C. Upon thawing, make working aliquots and store at -20°C. Avoid repeated freeze-thaws.
Stability    Stable for 6 months from date of receipt when stored as directed.
Quality Control    Tested by immunocytochemistry (Figure 1-2).
Recommended Usage    Dilute 1:100 for immunocytochemistry. Reported applications include Western blot, IHC, immunofluorescence and staining of tissue sections. For general protocol for performing immunocytochemistry on ES cells, see Technical Documents at the product page.
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