Stemgent® StainAlive™ DyLight™549 Mouse anti-Mouse/Human SSEA-1 Antibody

Stemgent® StainAlive™ DyLight™549 Mouse anti-Mouse/Human SSEA-1 Antibody详细信息：
Product Overview

 

Stemgent® StainAlive™ DyLight™ 549 Mouse anti-Mouse/Human SSEA-1 Antibody was screened on live, unfixed mouse embryonic stem (ES) cells by immunocytochemistry (ICC). This antibody was selected as the best conjugated SSEA-1 antibody available for EC/ES/iPS cells, utilizing fluorescent staining and real-time imaging without fixation or killing the cells. This formulation is useful to directly visualize the reprogramming process to iPS colonies. For use on live cells, this antibody has been specifically formulated with low endotoxin and no sodium azide and is confirmed to be free of bacteria, fungi, mycoplasma and mouse virus as tested. The live ES cell morphology, proliferation and expression of pluripotency markers did not change after staining with this antibody. The MC-480 antibody reacts with mouse and human stage-specific embryonic antigen-1 (SSEA-1), an antigenic epitope defined as a Lewis X carbohydrate structure. It is expressed on murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES), and murine and human germ cells. It is widely used as positive surface marker for mouse undifferentiated stem cells and negative surface marker for human undifferentiated stem cells. Expression is down-regulated following differentiation of murine EC and ES cells, while the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression. The SSEA-1 molecule is associated with cell adhesion, migration and differentiation.

Stemgent® StainAlive™ DyLight™549 Mouse anti-Mouse/Human SSEA-1 AntibodyProduct Specifications

Immunogen    Mouse F9 Teratocarcinoma Stem Cells (X-irradiated)
Reactivity    Mouse
Clone    MC-480
Isotype    Mouse IgM, κ
Formulation    Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 1 EU/μg of the protein (< 0.1 ng/μg of the protein) as determined by the LAL assay. It is free from bacteria, fungi, mycoplasma and mouse virus as tested.
Preparation    The antibody was purified by affinity chromatography, and conjugated with DyLight™ 549 under optimal conditions. The solution is free of unconjugated dye and free antibody.
Storage    Store at 4°C. Do not freeze. Light sensitive. This formulation contains no preservatives. Handle under aseptic conditions.
Stability    Stable for 6 months from date of receipt when stored as directed.
Quality Control    Tested by immunocytochemistry (ICC).
Recommended Usage    Dilute 1:100 for ICC. For general protocols for performing viable cell ICC directly on stem cells, see Technical Documents on the Stemgent® StainAlive™ DyLight™ 549 Mouse anti-Mouse/Human SSEA-1 Antibody product page.
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