Description |  | Phospho-specific polyclonal anti-peptide antibody to calcium/calmodulin kinase II P-Thr286 (CAMKII) |
Form |  | Affinity Purified Antibody |
Host |  | Rabbit |
Species Reactivity |  | Calcium/Calmodulin Kinase II [Thr286] Polyclonal Antibody, Phospho-Specific Rat |
[Ab] |  | 0.1 mg/mL |
Specificity |  | This antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr286 of CAM kinase II. It is specific for Mr 60 and 50 kD protein phosphorylated at Thr286. The labeling of the CAM K II bands in a western blot or rat brain is blocked by the phosphopeptide but not by the dephosphopeptide. This antibody is purified by sequential chromatography on Protein A and phospho and dephospho peptide affinity columns. |
Uses |  | Calcium/Calmodulin Kinase II [Thr286] Polyclonal Antibody, Phospho-Specific These antibodies are effective in immunoblotting, immunohistochemistry, immunoprecipitation and ELISA. |
Suggested Working Dilution |  | The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval. - Western blot: 1:1,000*
- Immunohistochemistry: 1:1,000
- Immunoprecipitation: 1:1,000
- ELISA: 1:1,000
|
Storage |  | Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C. |
Warranty/Conditions |  | Covance products may not be resold or modified for resale without prior written approval. |