Purified anti-mouse CD3 纯化的小鼠CD3抗体_详细资料

产品信息 供应商：上海恒斐生物科技有限公司数量：现货英文名： Purified anti-mouse CD3 规格： 50 ug Clone 17A2 Isotype Rat IgG2b, κ Reactivity Mouse Immunogen γδTCR-positive T-T hybridoma D1 Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Preparation The antibody was purified by affinity chromatography. Concentration 0.5mg/ml Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Applications FC - Quality tested IHC, IP - Reported in the literature Application Notes The 17A2 antibody recognizes ε/γ (but not ε/δ) of the CD3 complex. The 17A2 antibody can induce T cell activation and has been reported to deplete CD3+ cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, complement-mediated cytotoxicity1,3, immunohistochemical staining of acetone-fixed frozen sections1,4, in vitro stimulation of T cells1 and depletion of CD3+ cells in vivo2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 100208). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100238) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg). Application References 1. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (IP, IHC, Activ, CMCD) 2. Mysliwietz J, et al. 1992. Blood 80:2661. (Deplete) 3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (CMCD) 4. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC) 5. Zan H, et al. 2005. EMBO J. 24:3757. 6. Morgado P, et al. 2011. Infect Immun. 79:4401. PubMed 7. Xiao J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:386. PubMed " Description CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. Storage The antibody solution should be stored undiluted at 4°C. 温馨提示：不可用于临床ZL。 资料下载： Purified anti-mouse CD3.pdf 附件下载 (下载 0 次) 请 [登录] 后再下载!

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Clone	17A2
Isotype	Rat IgG2b, κ
Reactivity	Mouse
Immunogen	γδTCR-positive T-T hybridoma D1
Formulation	Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation	The antibody was purified by affinity chromatography.
Concentration	0.5mg/ml
Recommended Usage	Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Applications	FC - Quality tested IHC, IP - Reported in the literature
Application Notes	The 17A2 antibody recognizes ε/γ (but not ε/δ) of the CD3 complex. The 17A2 antibody can induce T cell activation and has been reported to deplete CD3+ cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, complement-mediated cytotoxicity1,3, immunohistochemical staining of acetone-fixed frozen sections1,4, in vitro stimulation of T cells1 and depletion of CD3+ cells in vivo2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 100208). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100238) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References	1. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (IP, IHC, Activ, CMCD) 2. Mysliwietz J, et al. 1992. Blood 80:2661. (Deplete) 3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (CMCD) 4. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC) 5. Zan H, et al. 2005. EMBO J. 24:3757. 6. Morgado P, et al. 2011. Infect Immun. 79:4401. PubMed 7. Xiao J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:386. PubMed "
Description	CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
Storage	The antibody solution should be stored undiluted at 4°C.