In Situ Cell Death AP 原位细胞凋亡AP标记 现货

上海妍琦生物科技有限公司是一家面向生命科学领域,专业经营进口胎牛血清、细胞因子、ELISA试剂盒等实验室试剂、化工产品、实验室消耗品、实验室耗材以及实验室器材。，产品领域：分子生物学、细胞生物学、细菌学、遗传学、免疫学、生物化学、蛋白质学等领域,致力于为ZG生命科学研究领域的客户提供ZY质的产品、ZX进的技术和最专业的服务。主要经营进口试剂品Pai有：Roche 、 GE 、 SIGMA 、  abcam  、Thermo、  Peprotech  、  Cayman 、  BioLegend 、  Bio-Rad 、 EnZo 、 CST 、 Life、BD  eBioscience  STANTCRUZ  Millipore  RD  等，耗材品Pai有：BIOHIT 、  Corning、BRAND 等。

In Situ Cell Death AP,原位细胞死亡AP标记
11684809910,1 kit (for up to 50 tests)

Sample material: Cytospin and cell smear preparations, adherent cells grown on slides, and frozen and paraffin-embedded tissue sections.
Background Information
Widely used methods to determine apoptosis include the analysis of the genomic DNA by agarose-gel electrophoresis and DNA fragmentation assays based on 3H-thymidine and, alternatively, 5-Bromo-2'-deoxy-uridine. The methods involve the separation of fragmented, low molecular-weight DNA from unfragmented, high molecular-weight DNA in a given cell population. Thus, these methods do not provide information about the fate of an individual cell in a given cell population or, particularly, in tissue sections. Alternatively, individual apoptotic cells may be microscopically recognized because of the characteristic appearance of nuclear chromatin condensation and fragmentation, but this method is subjective and limited to a relatively narrow time window when the morphological changes are at a maximum.
The hallmark of apoptosis is DNA degradation, which in early stages is selective to the internucleosomal DNA linker regions. The DNA cleavage may yield double-stranded and single-stranded DNA breaks (nicks). Both types of breaks can be detected by labeling the free 3'-OH termini with modified nucleotides (e.g., biotin-dUTP, DIG-dUTP, fluorescein-dUTP) in an enzymatic reaction. The enzyme terminal deoxynucleotidyl transferase (TdT) catalyzes the template-independent polymerization of deoxyribonucleotides to the 3'-end of single- and double-stranded DNA. This method has also been termed TUNEL (TdT-mediated dUTP-X nick end labeling). Alternatively, free 3'-OH groups may be labeled using DNA polymerases by the template-dependent mechanism called nick translation. However, the TUNEL method is considered to be more sensitive and faster.

Contents

    Enzyme Solution (TdT), 5 vials
    Label Solution (fluorescein-dUTP), 5 vials
    Converter AP (anti-fluorescein antibody-AP), ready-to-use

11684809910	In Situ Cell Death AP	1 kit (50 tests)	459130-BR	6149
11684817910	In Situ Cell Death POD	1 kit (50 tests)	459130-BR	6378
11774425001	Cell Death Detection ELISA^PLU	1 kit (96 tests)	459130-BR	4448
4906845001	PhosStop, 10 Tablets	10 tablets (each tablet for 10 ml)	459130-BR	1322
10109886001	Trypsin Inhibitor (STI), 50 mg	50 mg (non-sterile)	459130-BR	754
10874515001	Bestatin, 10mg	10 mg	459130-BR	2868
11585681001	E-64	25 mg	459130-BR	6954
11921673001	Western Blocking Reagent, 100	100 ml (10 blots, 100 cm2)	459130-BR	958
10799068001	Interleukin-2,human(hIL-2),rec	10,000 U (5 µg, 50 ml)	459130-BR	3683
11011456001	Interleukin-2,human(hIL-2), re	10,000 U (5 µg, 1 ml)	459130-BR	3683