兔抗人激活因子of 90 kDa热休克蛋白ATP酶同源物 1多克隆抗体;Bintin 标记

• 产品类型:
  Primary Antibody>Polyclonal Antibody
• 产品名称:
  兔抗人激活因子of 90 kDa热休克蛋白ATP酶同源物 1多克隆抗体;Bintin 标记
• 英文名称:
  Rabbit anti-human Activator of 90 kDa heat shock protein ATPase homolog 1 polyclonal Antibody;Biotin conjugated
• 兔抗人激活因子of 90 kDa热休克蛋白ATP酶同源物 1多克隆抗体;Bintin 标记
• 免疫原:
  Recombinant human Activator of 90 kDa heat shock protein ATPase homolog 1 protein
• 宿主:
  Rabbit
• 种属反应性:
  Human
• 应用范围:
  WB;ELISA
• 背景:
  Cochaperone that stimulates HSP90 ATPase activity By similarity. May affect a step in the endoplasmic reticulum to Golgi trafficking.
• 克隆类型:
  Polyclonal
• 抗体亚型:
  IgG
• 纯化方式:
  Caprylic Acid Ammonium Sulfate Precipitation
• 标记方式:
  Biotin conjugated
• 保存缓冲液:
  Preservative: 0.03% Proclin 300
  Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• 产品提供形式:
  Liquid
• 储存条件:
  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
• 参考文献:
  [1] "Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning."Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W.,
• 图片:
  Western blot

  

  All lanes : Activator of 90 kDa heat shock protein ATPase homolog 1 antibody at at 2 µg/ml
  
  Lane 1 : EC109 whole cell lysate
  Lane 2 : 293T whole cell lysate

   

  Secondary
  Goat polyclonal to Rabbit IgG at 1/15000 dilution

   

  Predicted band size : 37 kDa

  Observed band size: 45kDa

  Why is the actual band size different from the predicted?

  Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include...

  路 post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein

  路 post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases

  路 splice variants - alternative splicing may create different sized proteins from the same gene

  路 relative charge - the composition of amino acids (charged vs non-charged)

  路 multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.

   
• 说明书:
   
• Accession No.:
  O95433
• 兔抗人激活因子of 90 kDa热休克蛋白ATP酶同源物 1多克隆抗体;Bintin 标记
• 规格:
  100μg
• 研究领域:
  Neuroscience
•  

抗体的异质性：抗体的组成极为复杂，是由成千上万、多种多样的免疫球蛋白(Ig)分子所组成。这些Ig分子在形状、大小、结构以及氨基酸的组成和排列上，既相似，又有差别。由于有差别，它们的电泳活性就有很大的变化。 
抗体的主要功能是与抗原(包括外来的和自身的)相结合，从而有效地清除侵入机体内的微生物、寄生虫等异物，抗体（antibody）是一种应答抗原产生的、可与抗原特异性结合的蛋白质。兔抗人激活因子of 90 kDa热休克蛋白ATP酶同源物 1多克隆抗体;Bintin 标记hz-3933R    CACH6L型钙通道蛋白a1亚型6抗体
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hz-15112R    C20orf6220号染色体开放阅读框62抗体
hz-4262R    cIAP1凋亡YZ因子1抗体
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hz-6187R    C1 Inactivator酯酶YZ蛋白C1IN抗体
hz-6277R    CYP2W1细胞色素P450 2W1抗体
hz-6278R    Choline dehydrogenase胆碱脱氢酶抗体
hz-5764R    CIAPIN1细胞因子诱导凋亡YZ因子1
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hz-6226R    CT13肿瘤/睾丸抗原13抗体
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hz-6620R    CRHR2促肾上腺皮质释放激素受体2抗体
hz-9694R    C20orf13220号染色体开放阅读框132抗体
hz-4595R    Collagen IVIV型胶原蛋白/4型胶原蛋白/胶原蛋白4抗体
hz-4247R    CHRNB2型乙酰胆碱受体B2抗体
hz-15114R    C20orf70 20号染色体开放阅读框70抗体
hz-5911R    Cyclophilin B亲环蛋白（亲环素）PPIB抗体
hz-15287R    C8ORF348号染色体开放阅读框34抗体
hz-6678R    M CadherinM钙粘附分子抗体
hz-6655R    Cryopyrin细胞凋亡诱导蛋白NALP3抗体
hz-6656R    Cyclin B2周期素B2抗体
hz-6658R    Calumenin钙腔蛋白
hz-15241R    C6orf2226号染色体开放阅读框222抗体
hz-5833R    Cornulin热休克蛋白58抗体
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