- 产品类型:
Primary Antibody>Polyclonal Antibody
- 产品名称:
兔抗人激活因子of 90 kDa热休克蛋白ATP酶同源物 1多克隆抗体;FITC标记
- 英文名称:
Rabbit anti-human Activator of 90 kDa heat shock protein ATPase homolog 1 polyclonal Antibody;FITC conjugated
- 兔抗人激活因子of 90 kDa热休克蛋白ATP酶同源物 1多克隆抗体;FITC标记
- 免疫原:
Recombinant human Activator of 90 kDa heat shock protein ATPase homolog 1 protein
- 宿主:
Rabbit
- 种属反应性:
Human
- 应用范围:
WB;ELISA
- 背景:
Cochaperone that stimulates HSP90 ATPase activity By similarity. May affect a step in the endoplasmic reticulum to Golgi trafficking.
- 克隆类型:
Polyclonal
- 抗体亚型:
IgG
- 纯化方式:
Caprylic Acid Ammonium Sulfate Precipitation
- 标记方式:
FITC conjugated
- 保存缓冲液:
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
- 产品提供形式:
Liquid
- 储存条件:
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
- 参考文献:
[1] "Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning."Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W.,
- 图片:
Western blot
All lanes : Activator of 90 kDa heat shock protein ATPase homolog 1 antibody at at 2 µg/ml
Lane 1 : EC109 whole cell lysate
Lane 2 : 293T whole cell lysate
Secondary
Goat polyclonal to Rabbit IgG at 1/15000 dilution
Predicted band size : 37 kDa
Observed band size: 45kDa
Why is the actual band size different from the predicted?
Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include...
路 post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein
路 post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases
路 splice variants - alternative splicing may create different sized proteins from the same gene
路 relative charge - the composition of amino acids (charged vs non-charged)
路 multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.
- 说明书:
Direct ELISA ProtocolIndirect ELISA ProtocolSandwich ELISA ProtocolWestern Blotting Protocol
- Accession No.:
O95433
- 兔抗人激活因子of 90 kDa热休克蛋白ATP酶同源物 1多克隆抗体;FITC标记
- 规格:
100μg
- 研究领域:
Neuroscience
-
抗体的异质性：抗体的组成极为复杂，是由成千上万、多种多样的免疫球蛋白(Ig)分子所组成。这些Ig分子在形状、大小、结构以及氨基酸的组成和排列上，既相似，又有差别。由于有差别，它们的电泳活性就有很大的变化。
抗体的主要功能是与抗原(包括外来的和自身的)相结合，从而有效地清除侵入机体内的微生物、寄生虫等异物，抗体（antibody）是一种应答抗原产生的、可与抗原特异性结合的蛋白质。
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