碳酸酐酶Ⅻ(CA12)重组蛋白适用生物 Rattus norvegicus (Rat，大鼠)
碳酸酐酶Ⅻ(CA12)重组蛋白产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 19.0kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
Recombinant Carbonic Anhydrase XII (CA12)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Rattus norvegicus (Rat) |
| Product No. | RPD079Ra01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 19.0kDa |
| Predicted isoelectric point | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Subcellular Location | n/a |
| Residues | Leu154~Ser307 (Accession # A2IBE2) with N-terminal His-Tag |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF- LYSDFGS ASDKSEGLAV LAVLIEIGSV NPSYDKIFSH LQHVKYKGQQ VLIPGFNIEE LLPESPGEYY RYEGSLTTPP CYPTVLWTVF RNPVQISQEQ LLALETALYF THMDDPSPRE MVNNFRQVQK FDERLVYISF RQGLLTDTGL SLGIILS
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
碳酸酐酶Ⅻ(CA12)重组蛋白Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
碳酸酐酶Ⅻ(CA12)重组蛋白Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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