Human Protein inhibitor of activated STAT3,PIAS3 ELISA Kit使用说明书本

Human Protein inhibitor of activated STAT3,PIAS3 ELISA Kit使用说明书本货号：GD-QX0292
人STAT3蛋白YZ分子(PIAS3)ELISA试剂盒
现货供应人STAT3蛋白YZ分子ELISA试剂盒,促销人(PIAS3)ELISA试剂盒,elisakit

高品质Elisa试剂盒：人STAT3蛋白YZ分子(PIAS3)ELISA试剂盒
Operation steps

标准品的稀释与加样：在酶标包被板上设标准品孔10孔，在DY、第二孔中分别加标准品100μl，然后在DY、第二孔中加标准品稀释液50μl，混匀；然后从DY孔、第二孔中各取100μl分别加到第三孔和第四孔，再在第三、第四孔分别加标准品稀释液50μl，混匀；然后在第三孔和第四孔中先各取50μl弃掉，再各取50μl分别加到第五、第六孔中，再在第五、第六孔中分别加标准品稀释液50ul，混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中，再在第七、第八孔中分别加标准品稀释液50μl，混匀后从第七、第八孔中分别取50μl加到第九、第十孔中，再在第九第十孔分别加标准品稀释液50μl，混匀后从第九第十孔中各取50μl弃掉。 The samples of each hole after dilution were 50 L, and the concentrations were 90 ng/L, 60 ng/L, 30 ng/L, 15 ng/L, 7.5 ng/L, res
Add sample: respectively, the blank hole (blank control hole without the sample and the enzyme label, and the remaining steps of the same), to sample the sample hole. In the enzyme standard coated plate to be tested on a sample hole Zhongxian sample dilution of 40 g l, and then to be measured is added 10 mu l of sample (sample final dilution degrees for 5 times). The sample is added to the bottom of the hole of the plate, and the wall is not touched, and the mixing is lightly and evenly..
Temperature Education: 37 minutes after the closure of the sealing plate and the temperature of 30.
With liquid: 30 (48T 20 times) concentrated detergent liquid with distilled water 20 (48T 30 times) after dilution.
Washing: be careful torn off the seal plate membrane, discard liquid, drying, washing liquid to fill each hole, standing for 30 seconds after the discard, repeat 5 times, pat dry.
Add enzyme: 50 L per hole, except for blank pore.
Wen Yu: operation with 3.
Wash: operation with 5.
Color: every hole first add color reagent A50 L, then add color agent B50 L, gently shake mix, 37 degrees Celsius color 15 minutes.
Termination: 50 L per hole plus end of liquid, terminate the reaction (at this point blue turns yellow).
Determination: the blank absorbance at 450nm air conditioning zero, in order to measure the hole (OD). Determination should be carried out within 15 minutes after the addition of the termination of the liquid.

Notes:
The kit is removed from the refrigeration environment for minutes at room temperature for a balanced 15-30 minutes after the enzyme is coated with the plate, such as not used up, the slab should be stored in the sealing bag.
Washing buffer will crystallization, heated the water solubilization dilution, washing does not affect the results.
Each step sample adding sample should use the sample device, and often to check its accuracy, in order to avoid testing error. A sample within 5 mins, ifthenumberofsampleismuch, recommend to use volley like.
Please do the standard curve at the same time, and it is best to do the hole. Such as samples to be measured matter content is too high (the sample od is bigger than the first standard hole hole OD), please first sample dilution multiples (n times) were measured and calculated please then multiplied by the total dilution ratio (n * * 5).
Closure plate membrane for disposable use, to avoid cross contamination.
Substrate please keep the light from the light..
Strictly according to the operation of the manual, the test results must be determined by the reader of the reader's reading.
All samples, washing liquid and all kinds of wastes should be treated by the infective substances.This reagent not mix different batches..
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10 if there is a different from the instruction of the English language, the English instruction manual shall prevail..
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Calculation:.
To the standard concentration as the abscissa, OD value as the ordinate, draw the standard curve on coordinate paper, according to the OD value of samples from the standard curve found corresponding concentration; multiplied by the dilution multiple; or with the standard concentration and the OD value calculated straight line regression equation of the standard curve, the sample OD value in the equation, calculate the sample concentration, multiplied by the dilution factor is the actual concentration of the sample..

Kit performance:.
1 the correlation coefficient R value of linear regression and the expected concentration of sample was 0.92 above..
The 2 batch and the batch should be less than 9% and 15% respectively..
Detection range:.
-100 ng/L 3ng/L.
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Save condition and its validity:.
1 reagent kit preservation: 2-8..
2 validity: 6 months.
Human Protein inhibitor of activated STAT3,PIAS3 ELISA Kit使用说明书本