产品名称
Other NotesBased on miRBase V19 Mature IDGeneral descriptionIndividual lenti microRNA inhibitors are designed using a proprietary algorithm, which is based on the work of Haraguchi, T, et al. and in collaboration with Dr. Hideo Iba, University of Tokyo. This algorithm utilizes the tough decoy (TuD) design. miRNA are known to regulate gene expression in a variety of manners, including translational repression, mRNA cleavage and deadenylation. The lentiviral microRNA Inhibitors are cloned into the TRC2-pLKO-puro vector. Co-transfection of this vector into the appropriate cell line with compatible packaging plasmids produces viral particles that can be used to transduce mammalian cells. Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory Element2 (WPRE) is included, allowing for enhanced expression of transgenes delivered by lentiviral vectors. This lentiviral vector also carries a puromycin resistance gene for selection of cells.
• Allows for potent inhibition of the desired miRNA
• Lentiviral delivery format allows for efficient delivery of the inhibitor into a wide variety of cell types
• Enables long-term inhibition without repeat transfection
法律信息MISSION is a registered trademark of Sigma-Aldrich Co. LLC
性质
Related CategoriesHuman Lenti microRNA Inhibitors,MISSION Lenti microRNA Inhibitors,hsa-miR-1501 to hsa-miR-4000,分子生物学,功能基因组和RNAi
更多...
application(s) capture ELISA: 106 TU/mL using p24mature sequence GCUCGGACUGAGCAGGUGGGSanger mature/minor accession no. MIMAT0018191Sanger microRNA accession no. MI0016423shipped in dry icestorage temp. −70°C
温馨提示:不可用于临床ZL。