Rat Big Endothelin,Big ET ELISA Kit使用原理

Rat Big Endothelin,Big ET ELISA Kit使用原理
大鼠大内皮素(BigET)ELISA试剂盒
货号:FK-QZ2105
规格：96T/48T
Rat Big Endothelin,Big ET ELISA Kit使用原理The kit was a solid phase sandwich enzyme-linked immunosorbent assay (ELISA), and the AVP concentrations of the standard samples and unknown concentrations of samples were detected by the addition of microporous enzyme plates.. At the same time, the AVP and the biotin labeled antibodies were incubated at the same time.. After washing, the addition of Pro labeled HRP. After the warming and washing, the removal of the non binding enzyme conjugate, and then added to the substrate a, B, and the enzyme conjugate at the same time. Produce color. The color depth and the concentration of AVP in the sample was proportional to the concentration of the sample..

Operation notes
1 reagent should be stored in the label manual, before using to room temperature. The standard after dilute dilute should be discarded, do not save.
2 the experimental plate should be immediately put back in the bag, seal the preservation, lest metamorphism.
3 no other reagents should be packaged or well. Do not mix different batches of reagents. Use before the shelf.
4 use a disposable suction head lest cross contamination, draw the termination liquid and substrate B, a liquid, to avoid using the metal part of the sample.
5 use clean plastic containers to configure the washing liquid. All samples of ingredients and mix well before use in the kit.
6 wash the enzyme plate should be fully dry, do not absorb water paper directly into the enzyme labeled pores in the water absorption.
7 substrate a should be volatile, to avoid prolonged open lid. Substrate B is sensitive to light, avoiding prolonged exposure to light. To avoid contact with hand, toxic. After the experiment, read the OD value immediately..
8 add reagents should be consistent in order, to ensure that all reaction plate hole incubation time.
9 to carry out the temperature fertility operation according to the time, the amount and the order of the liquid added in the instruction manual.

Kit performance:
1 the correlation coefficient r value of linear regression and the expected concentration of sample was 0.92 above.
The 2 batch and the batch should be less than 9% and 15% respectively.
Detection range:
1.5 -90 g/ml g/ml
Save condition and its validity:
1 reagent kit preservation: 2-8.
2 validity: 6 months
大鼠大内皮素(BigET)ELISA试剂盒

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