视网膜母细胞瘤蛋白1(RB1)重组蛋白

视网膜母细胞瘤蛋白1(RB1)重组蛋白 Recombinant Retinoblastoma Protein 1 (RB1)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species    Homo sapiens (Human)
视网膜母细胞瘤蛋白1(RB1)重组蛋白
Source    Prokaryotic expression
Host    E.coli
Purity    > 95%
UOM    50ug
Predicted Molecular Mass    49.2kDa
Predicted isoelectric point    7.7
Applications    SDS-PAGE; WB; ELISA; IP.
Endotoxin Level    <1.0EU per 1µg (determined by the LAL method)
视网膜母细胞瘤蛋白1(RB1)重组蛋白
Subcellular Location    Nucleus
Residues    Gln639~Thr778 (Accession # P06400) with two N-terminal Tags, His-tag and GST-tag
Formulation    Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-QK PLKSTSLSLF YKKVYRLAYL RLNTLCERLL SEHPELEHII WTLFQHTLQN EYELMRDRHL DQIMMCSMYG ICKVKNIDLK FKIIVTAYKD LPHAVQETFK RVLIKEEEYD SIIVFYNSVF MQRLKTNILQ YASTRPPT
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
视网膜母细胞瘤蛋白1(RB1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.hz-7098R    CLIC4细胞内氯离子通道蛋白4抗体
hz-6373R    CGR19细胞生长调控蛋白19/环指蛋白197抗体
hz-7102R    CCDC139卷曲螺旋结构域蛋白139抗体
hz-6375R    CHMP1A染色体修饰蛋白1抗体（金属蛋白酶1）
hz-11812R    CAPS1钙依赖分泌激活蛋白1抗体
hz-11814R    CBLN1小脑肽1抗体
hz-11815R    CBLN2小脑肽2抗体
hz-11816R    CBLN4小脑肽4抗体
hz-11817R    CCM2脑海绵状血管畸形蛋白2抗体
hz-11818R    CELSR2表皮生长因子样蛋白2抗体
hz-11819R    CHRDL1脊索生成素样蛋白1抗体
hz-11820R    CHURC1神经诱导胚胎发育相关蛋白Churchill抗体
hz-11821R    CRMP3二氢嘧啶酶相关蛋白3抗体
hz-11427R    CRSP9转录激活蛋白CRSP9抗体
hz-9576R    Copine-6神经网蛋白CopineVI抗体
hz-11416R    Cpt1c脑型肉碱棕榈酰转移酶1C抗体
hz-6949R    Factor B补体因子B抗体
hz-10169R    CHRNA1乙酰胆碱受体前体蛋白AChRα1抗体
hz-4527R    CSFV Envelope glycoprotein E2猪瘟病毒包膜糖蛋白E2抗体
hz-11823R    CSDC2冷休克结构域蛋白C2抗体
hz-11830R    CA10碳酸酐酶10抗体
hz-6953R    CD62LL选择素抗体
hz-4963R    C-Myc致癌基因C-Myc抗体
hz-4975R    CCK8胆囊收缩素8抗体
hz-10009R    E cadherin上皮钙粘附分子抗体
hz-4898R    CITED1结合转化激活因子CITED1抗体
hz-11929R    Collagen IIⅡ型胶原α1蛋白/软骨钙素抗体
hz-8187R    CSMD2CSMD2蛋白抗体
hz-8190R    CSMD3CSMD3蛋白抗体
hz-8191R    CSTP1钙调神经磷酸酶CSTP1抗体
hz-8192R    CTAGE6CTAGE6蛋白抗体
hz-8193R    CTAGE4皮肤T细胞淋巴瘤相关抗原4抗体
hz-8014R    CLDND2膜蛋白CLDND2抗体
hz-6409R    CHORDC1含半胱氨酸和组氨酸丰富域蛋白1抗体