乙酰基赖氨酸抗体，Anti-acetyl Lysine抗体

背景介绍：In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo.

别名：Ac-lysine; acetyl Lysine; Lysine.

乙酰基赖氨酸抗体，Anti-acetyl Lysine抗体简单介绍：

产品编号：BYK-8850R

英文名称：Anti-acetyl Lysine
中文名称：乙酰基赖氨酸抗体

产品规格：50ul；100ul；200ul；1ml，品质保证

抗体来源 Rabbit or Mouse

克隆类型 Polyclonal or Monoclonal

交叉反应、产品应用 （具体交叉反应与产品应用请或添加客服直接索取）

性    状 Lyophilized or Liquid
浓    度 1mg/ml

乙酰基赖氨酸抗体，Anti-acetyl Lysine抗体产品信息：

博研生物小课堂：

常用激光器对应荧光素的选择：

蓝色激光器荧光素（488nm）：Alexa Fluor 488、FITC、PerCP、PerCP-Cy5.5、PE、PE-Cy5.5、PE-Cy5、PE-Cy7

红色激光器荧光素（633nm）：APC、Cy5、APC-Cy5.5、APC-Cy7、Alexa Fluor 647

黄色激光器荧光素（561nm）：PE、PE-Cy5.5、PE-Cy5、PE-Cy7、Cy3、Alexa Fluor 555、Alexa Fluor 594

免疫组化结果的判断

对免疫组化结果的判断应持科学的慎重态度，要准确判断阳性和阴性，排除假阳性和假阴性结果，必须严格对照实验，对新发现的阳性结果，除有对照实验结果之外，应进行多次重复实验，可用几种方法进行验证。必须学会判断特异性染色和非特异性染色，对初学者更为重要，否则会得出不科学的结论。特异性染色和非特异性染色的鉴别点主要在于特异性反应产物常分布于特定的部位。如胞浆内，也有分布在细胞核和细胞表面的，即具有结构性。特异性染色表现为在同一切片上呈现不同程度的阳性染色结果。非特异性染色表现为无一定的分布规律，常呈某一部位成片的均匀着色，细胞和周围的结缔组织均无区别的着色，或结缔组织呈现很强的染色。非特异性染色常出现在干燥切片的边缘，有刀痕或者折叠的部位。在过大的组织块，ZX固定不良也会导致非特异性染色。有时可见非特异性染色和特异性染色同时存在，由于过强的非特异性染色背景不但影响对特异性染色结果的观察和记录，而且令人对其特异性结果产生怀疑。

免疫组化的呈色深浅可反映抗原存在的数量，可作为定性、定位和定量的依据。

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