背景介绍:This gene encodes an enzyme that hydrolyzes terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. Its activity is necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover and it is member of family 38 of glycosyl hydrolases. The full length protein is processed in two steps. First, a 49 aa leader sequence is cleaved off and the remainder of the protein is processed into 3 peptides of 70 kDa, 42 kDa (D) and 13/15 kDa (E). Next, the 70 kDa peptide is further processed into three peptides (A, B and C). The A, B and C peptides are disulfide-linked. Defects in this gene have been associated with lysosomal alpha-mannosidosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
别名:EC 3.2.1.24; Laman; Lysosomal acid alpha mannosidase; Lysosomal alpha mannosidase; MANB; Mannosidase alpha class 2B member 1; Mannosidase, alpha B; Mannosidase, alpha B, lysosomal.
溶酶体α-甘露糖苷酶抗体,Anti-MAN2B1抗体简单介绍:
产品编号:BYK-18647R
英文名称:Anti-MAN2B1
中文名称:溶酶体α-甘露糖苷酶抗体
产品规格:50ul;100ul;200ul;1ml,品质保证
抗体来源 Rabbit or Mouse
克隆类型 Polyclonal or Monoclonal
交叉反应、产品应用 (具体交叉反应与产品应用请或添加客服直接索取)
性 状 Lyophilized or Liquid
浓 度 1mg/ml
溶酶体α-甘露糖苷酶抗体,Anti-MAN2B1抗体产品信息:
Western Blotting 实验步骤概述
1、 组织取材:组织块称重
2、 利用液氮、研钵粉碎组织块
3、 加入RIPA缓冲液(每克组织3ml RIPA),PMSF(每克组织30μl,10 mg/ml PMSF),
利用Polytron进一步匀浆(15,000转/分*1分钟)维持4℃
4、 加入PMSF(每克组织30μl,10 mg/ml PMSF),冰上孵育30分钟
5、 移入离心管4℃,约20,000g(约15,000转)15分钟
6、 上清液为细胞裂解液可分装-20℃保存
7、 进行Bradford比色法测定蛋白质浓度
8、 取相同质量的细胞裂解液(体积*蛋白质浓度),并加等体积的2×电泳加样缓冲液
9、 沸水浴中3分钟
10、上样
11、电泳(浓缩胶20mA,分离胶35mA)
12、电转膜仪转膜(100mA 40分钟)
13、膜用丽春红染色,胶用考马斯亮蓝染色
14、Westernblot 试剂盒显色
15、分析比较记录
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