背景介绍:Misfolded glycoproteins are deglycosylated by the peptide N-glycanase during the degradation process. Free oligosaccharides released by N-glycanase are catabolized by cytosolic MAN2C1, also designated alpha-mannosidase 2C1. MAN2C1, a member of the glycosyl hydrolase 38 family, can cleave alpha 1,2-linked, alpha 1,3-linked and alpha 1,6-linked mannose residues and is stimulated by cobalt. The furanose analogs, swainsonine (SW) and 1,4-dideoxy-1,4-imino-d-mannitol (DIM), are known inhibitors of MAN2C1. The inhibition of MAN2C1 can enhance the adhesion of Jurkat T cells, showing a cytoskeletal rearrangement of the cells.
别名:alpha D mannoside mannohydrolase; alpha mannosidase 2C1; Alpha mannosidase 6A8B; Alpha-D-mannoside mannohydrolase; Alpha-mannosidase 2C1; MA2C1_HUMAN; Man2c1; MAN6A8; MANA; MANA1; Mannosidase alpha 6A8; Mannosidase alpha A cytoplasmic; Mannosidase alpha class 2C member 1.
甘露糖苷酶α胞浆抗体,Anti-MAN2C1抗体简单介绍:
产品编号:BYK-18649R
英文名称:Anti-MAN2C1
中文名称:甘露糖苷酶α胞浆抗体
产品规格:50ul;100ul;200ul;1ml,品质保证
抗体来源 Rabbit or Mouse
克隆类型 Polyclonal or Monoclonal
交叉反应、产品应用 (具体交叉反应与产品应用请或添加客服直接索取)
性 状 Lyophilized or Liquid
浓 度 1mg/ml
甘露糖苷酶α胞浆抗体,Anti-MAN2C1抗体产品信息:
Western Blotting 问题解答
背景高?
原因:
1.HRP浓度过高;
2.封闭无效;
3.不适合的封闭试剂;
4.洗涤不充分和洗涤液的量;
5.胶片曝光过度;
6.抗原或抗体的浓度太高。
解决:
1.稀释HRP至少10倍;
2.寻找合适的封闭方法;
3.选用其他的封闭试剂;
4.增加洗涤的时间,次数;
5.减少曝光时间;
6.稀释抗原或抗体。
7、蛋白质条带上有空泡影像?
原因:
1.蛋白质没有被充分转印;
2.膜没有预先浸湿;
3.在胶片和膜之间有气泡。
解决:
1.寻找合适的转移方法和条件;
2.在转印前充分浸湿;
3.在暴光之前赶走气泡。
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