Anti-TIAF1抗体;TGFB1诱导抗凋亡因子1抗体简单介绍:
产品编号:BYK-10702R
英文名称:Anti-TIAF1
中文名称:TGFB1诱导抗凋亡因子1抗体
别名:MAJN; TGFB1 induced anti apoptotic factor 1 (12 kDa TGF beta1 induced antiapoptotic factor); 12 kDa TGF-beta-1-induced antiapoptotic factor; TGFB1 induced anti apoptotic factor 1; TGFB1-induced anti-apoptotic factor 1; TIAF1_HUMAN; MAJN; SPR210; MYO18A; MYSPDZ; TGFB1 induced anti apoptotic factor 1.
背景介绍:TIAF-1 (TGF Beta 1-induced antiapoptotic factor 1), also known as MYO18A, MAJN or MYSPDZ, is a TGF Beta 1-induced anti-apoptotic factor that localizes to the nucleus and functions to inhibit the cytotoxic effects of TNF? TRADD and FADD. Two isoforms of mouse TIAF-1 exist and are designated MysPDZ-Alpha and MysPDZ-Beta. MysPDZ-Alpha consists of a KE-rich region, an N-terminal PDZ domain and a prevalent myosin homologous head region, while the MysPDZ-Beta isoform lacks the KE-rich region and PDZ domain. MysPDZ-Alpha is present in most tissues and is known to co-localize with the ER-Golgi complex and with membrane ruffles and filopodia. MysPDZ-Beta is expressed specifically in hematopoietic tissues and cell lines and shows dispersed localization in the cytoplasm.
产品规格:50ul;100ul;200ul;1ml,品质保证
抗体来源 Rabbit or Mouse
克隆类型 Polyclonal or Monoclonal
交叉反应、产品应用 (具体交叉反应与产品应用请或添加客服直接索取)
性 状 Lyophilized or Liquid
浓 度 1mg/ml
Anti-TIAF1抗体;TGFB1诱导抗凋亡因子1抗体产品信息:
SABC法
- 脱蜡、水化
- PBS洗2次各5分钟
- 用蒸馏水或PBS配制新鲜的3% H2O2 ,室温封闭5-10分钟,用蒸馏水洗3次
- 抗原修复
- PBS洗5分钟
- 滴加正常山羊血清封闭液,室温20分钟,甩去多余液体
- 滴加Ι抗50ul,室温静置1小时或4℃过夜或37℃1小时
- PBS洗3次各2分钟
- 滴加生物素化Ⅱ抗,20℃-37℃ 20分钟
- PBS洗3次各2分钟
- 滴加试剂SABC 20℃-30℃ 20分钟
- PBS洗4次各5分钟
- DAB显色:试剂盒或自配显色剂显色
- 脱水、透明、封片、镜检
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