Anti-TOE1抗体-早期生长应答蛋白1靶向蛋白1抗体简单介绍:
产品编号:BYK-18167R
英文名称:Anti-TOE1
中文名称:早期生长应答蛋白1靶向蛋白1抗体
别名:Target of EGR1 protein 1; target of EGR1, member 1; Toe1; TOE1_HUMAN.
背景介绍:TOE1 is a 510 amino acid long protein that belongs to the CAF1 family. It is a downstream target of EGR-1 and plays an important role mediating the inhibitory growth effect of EGR-1. EGR-1 directly binds the TOE1 promoter region and activates its expression. TOE1 induces the expression of TGF∫ and p21 and plays a role in cell cycle regulation and the inhibition of cell growth. The activity and nucleolar localization of TOE1 correlates with a G2 cell cycle phase delay which is likely due to an increased expression of p21. In addition, TOE1 may function via an interaction with p53 and possible modification to its activity. TOE1 is expressed at various levels in all adult tissues and specifically localizes to the nuclear speckles.
产品规格:50ul;100ul;200ul;1ml,品质保证
抗体来源 Rabbit or Mouse
克隆类型 Polyclonal or Monoclonal
交叉反应、产品应用 (具体交叉反应与产品应用请或添加客服直接索取)
性 状 Lyophilized or Liquid
浓 度 1mg/ml
Anti-TOE1抗体-早期生长应答蛋白1靶向蛋白1抗体产品信息:
石蜡切片免疫组化染色步骤
1、三步法(以SP试剂盒为例)
- 石蜡切片脱蜡至水
- 蒸馏水冲洗,PBS浸泡5分钟,如果采用抗原修复,可在此步后进行
- 3% H2O2 室温孵育5~10分钟,以消除内源性过氧化物酶的活性,PBS冲洗,2分钟×3次
- 5~10%正常山羊血清封闭,室温孵育10分钟。倾去血清,勿洗,滴加适当比例稀释的一抗或一抗工作液,37℃孵育1~2小时或4℃过夜
- PBS冲洗,2分钟×3次
- 滴加适当比例稀释的生物素标记二抗(1%BSA-PBS稀释),37℃孵育10~30分钟;或滴加第二代生物素标记二抗工作液,37℃或室温孵育10~20分钟
- PBS冲洗,2分钟×3次
- 滴加适当比例稀释的辣根酶标记链霉卵白素(PBS稀释),37℃孵育10~30分钟;或第二代辣根酶标记链霉卵白素工作液,37℃或室温孵育10~20分钟
- PBS冲洗,2分钟×3次
- 显色剂显色(DAB或AEC)
- 自来水充分冲洗,复染,脱水透明、封片
- 如果必要,可选用avdin封闭内源性生物素
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