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产品信息 IBEX Technologies代理，IBEX TechnologiesZG代理，IBEX Technologies上海代理，IBEX Technologies北京代理，IBEX Technologies广州代理，IBEX Technologies代理，IBEX Technologies Heparinase I - Diagnostic Grade Datasheet Synonyms Heparinase; heparin lyase; heparin eliminase Source Flavobacterium heparinum (recombinant) EC Number CAS_Number 4.2.2.7 9025-39-2 Catalyzed Reaction The enzyme cleaves selectively, via an elimination mechanism, highly sulfated polysaccharide chains containing 1-4 linkages between hexosamines and O-sulfated iduronic acid residues. The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm. The enzyme also cleaves the antithrombin III binding pentasaccharide domain in the heparin molecule. Substrate Specificity Heparin; heparan sulfate (specific activity with heparin is approx. 3 times higher than with heparan sulfate). Properties: Molecular weight: 42,508 Da Isoelectric point: 9.3 – 9.5 pH optimum for activity: 6.5 – 7.5 pH range for activity: 4 – 9 Optimal temperature range: 20 ° C – 37 ° C Purity ≥ 95 % by reversed phase HPLC analysis. Specific Activity >90-110 IU/mg using the following Unit definition.One international unit (IU) is defined as the amount of enzyme that will liberate 1.0 µmole unsaturated oligosaccharides from porcine mucosal heparin per minute at 30 ° C and pH 7.0. (One Unit (U) is also defined in other preparations as 1 U forms 0.1 µmol of unsaturated uronic acid per hour at 25° C and pH 7.5; 1 IU is equivalent to 600U). Stability Expiration is primarily established at 3 years after manufacturing. The Heparinase I is kept frozen at -70 ° C in aqueous buffers containing Sodium Phoshate or Sucrose 5% 欢迎下载下面的资料以了解此产品的相关信息！预询产品订购信息 上海普迈生物致力于提供zui高质量的产品和服务组织形态学研究与竞争力的产品 产品和服务,有相关产品的问题请直接 期货每周下午5点前发定单，正常到上海时间为3-4周 现市区2天内送到，外地以快递为准 敬请关注！ ： :www.pumaibio.com :pumaibio :（直接客户） （经销商） 促销etonbio、polysciences、Mattek、trevigen、Chromogenix、cyguns、IrvineScientific、Innovative Cell Technologies、enzo、novus、immunochemistry、eylabs、Polyplus、NIBSC、ALZET、hitobiotec、sutter instrument、mirus、StressMarq、Avanti、jena bioscience、Neuro Probe、tedpella、avanti、Rockland、CAYMAN、transgenomic、BioLegend、ambion、omicronbio、 Assaybiotech、chondrex、lc?laboratories、cosmobio、megazyme、NeoMarkers、BTI、eylabs、berryassoc、cellagentech、TOCRIS、Relia Tech、SouthernBiotech、Biosearch technology、Lumiprobe、StemCells?Inc、biovendor、NORGEN、alomone、jena?bioscience、Gracebio、Eurogentec、Gelcompany?、invitrogen、gene?bridges、MRC-Holland、laysanbio

Heparinase I - Diagnostic Grade Datasheet
Synonyms	Heparinase; heparin lyase; heparin eliminase
Source	Flavobacterium heparinum (recombinant)
EC Number CAS_Number	4.2.2.7 9025-39-2
Catalyzed Reaction	The enzyme cleaves selectively, via an elimination mechanism, highly sulfated polysaccharide chains containing 1-4 linkages between hexosamines and O-sulfated iduronic acid residues. The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm. The enzyme also cleaves the antithrombin III binding pentasaccharide domain in the heparin molecule.
Substrate Specificity	Heparin; heparan sulfate (specific activity with heparin is approx. 3 times higher than with heparan sulfate).
Properties:	Molecular weight: 42,508 Da Isoelectric point: 9.3 – 9.5 pH optimum for activity: 6.5 – 7.5 pH range for activity: 4 – 9 Optimal temperature range: 20 ° C – 37 ° C
Purity	≥ 95 % by reversed phase HPLC analysis.
Specific Activity	>90-110 IU/mg using the following Unit definition.One international unit (IU) is defined as the amount of enzyme that will liberate 1.0 µmole unsaturated oligosaccharides from porcine mucosal heparin per minute at 30 ° C and pH 7.0. (One Unit (U) is also defined in other preparations as 1 U forms 0.1 µmol of unsaturated uronic acid per hour at 25° C and pH 7.5; 1 IU is equivalent to 600U).
Stability	Expiration is primarily established at 3 years after manufacturing. The Heparinase I is kept frozen at -70 ° C in aqueous buffers containing Sodium Phoshate or Sucrose 5%

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