干扰素α21(IFNα21)重组蛋白

干扰素α21(IFNα21)重组蛋白 Recombinant Interferon Alpha 21 (IFNa21)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species    Homo sapiens (Human)
Product No.    RPG966Hu01
Source    Prokaryotic expression
Host    E.coli
Purity    > 95%
UOM    50ug
Predicted Molecular Mass    51.2kDa
Predicted isoelectric point    6.1
Applications    SDS-PAGE; WB; ELISA; IP.
Endotoxin Level    <1.0EU per 1µg (determined by the LAL method)
干扰素α21(IFNα21)重组蛋白 
Subcellular Location    Secreted
Residues    Asp25~Glu189 (Accession # P01568) with two N-terminal Tags, His-tag and GST-tag
Formulation    Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-DLPQTH SLGNRRALIL LAQMGRISPF SCLKDRHDFG FPQEEFDGNQ FQKAQAISVL HEMIQQTFNL FSTKDSSATW EQSLLEKFST ELNQQLNDLE ACVIQEVGVE ETPLMNVDSI LAVKKYFQRI TLYLTEKKYS PCAWEVVRAE IMRSFSLSKI FQERLRRKE
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
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