Linker For Activation of T Cells (LAT) 抗体

Linker For Activation of T Cells (LAT) 抗体抗原    
Linker For Activation of T Cells (LAT)
别名     LAT, lat, LAT1, pp36
适用    
 其他选择 小鼠, 大鼠
宿主    
 其他选择 小鼠
克隆类型 (克隆位点)    
单克隆 (LAT-1D1)
标记    
 其他选择 非结合性
应用范围    
 其他选择 免疫细胞化学（ICC）, 免疫沉淀（IP）, Western Blotting (WB)
Pubmed     找到1个引用
规格     0.1 mg
发货至     ZG (更改)
发货时间     23至29个工作日
产品编号 yb94420
产品细节 Linker For Activation of T Cells (LAT) 抗体
免疫原     Bacterially expressed full-length rat LAT
克隆位点     LAT-1D1
亚型     IgG2a
特异性     The antibody LAT-1D1 reacts with mouse and rat Linker for activation of T cells (LAT), a transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets.
无交叉反应     人
纯化方法     Purified from hybridoma culture supernatant by protein-A affinity chromatography.
纯度     > 95 % (by SDS-PAGE)
目标详细情况 Linker For Activation of T Cells (LAT) 抗体
别名     LAT
背景     LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.
研究领域     Signaling, Adaptive Immunity
使用细节 Linker For Activation of T Cells (LAT) 抗体
应用备注     Western Blotting: Recommended dilution: 5-10 µg/mL 
Positive control: mouse spleen

Working concentrations should be determined by the investigator.
限制     仅限研究用
贮存及处理 Linker For Activation of T Cells (LAT) 抗体
浓度     1 mg/mL
缓冲液     Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
储存液     Sodium azide
注意事项     WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项    Do not freeze.
储存条件     4 °C
储存方法     Store at 2-8 °C. Do not use after expiration date stamped on vial label.