硫酸肝素蛋白聚糖2(HSPG2)重组蛋白

硫酸肝素蛋白聚糖2(HSPG2)重组蛋白 Recombinant Heparan Sulfate Proteoglycan 2 (HSPG2)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species    Homo sapiens (Human)
Product No.    RPC748Hu01
Source    Prokaryotic expression
Host    E.coli
Purity    > 95%
UOM    50ug
Predicted Molecular Mass    27.0kDa
Predicted isoelectric point    6.0
Applications    SDS-PAGE; WB; ELISA; IP.
Endotoxin Level    <1.0EU per 1µg (determined by the LAL method)

Subcellular Location    Secreted, extracellular space, extracellular matrix, basement membrane.
Residues    Leu4149~Ser4391 (Accession # P98160) with N-terminal His-Tag
Formulation    Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-LR EPCLHGGTCQ GTRCLCLPGF SGPRCQQGSG HGIAESDWHL EGSGGNDAPG QYGAYFHDDG FLAFPGHVFS RSLPEVPETI ELEVRTSTAS GLLLWQGVEV GEAGQGKDFI SLGLQDGHLV FRYQLGSGEA RLVSEDPIND GEWHRVTALR EGRRGSIQVD GEELVSGRSP GPNVAVNAKG SVYIGGAPDV ATLTGGRFSS GITGCVKNLV LHSARPGAPP PQPLDLQHRA QAGANTRPCP S
硫酸肝素蛋白聚糖2(HSPG2)重组蛋白USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this 硫酸肝素蛋白聚糖2(HSPG2)重组蛋白protein is less than 5% within the expiration date under