SuperScript® III First-Strand Synthesis System 货号18080-051_详细资料

产品信息

数量：现货

内容物及储存（Contents and Storage） The SuperScript® III First-Strand Synthesis System for RT-PCR is supplied with the components listed in Table 1. Store at -20°C. Guaranteed stable for 6 months when properly stored.

品Pai（Brand）
SuperScript®

反应形式（Reaction Format） Separate Components

反转率酶（Reverse Transcriptase） SuperScript® III

Downstream Application（Downstream Application） RT-PCR (Endpoint),

最适反应温度（Optimal Reaction Temperature） 50° C

产品分类（Product Category）分析试剂盒(Assay Kit)

产品描述

参考文献(19)

品Pai介绍
The SuperScript® III First-Strand Synthesis System for RT-PCR provides all the necessary components for synthesis of high-quality first-strand cDNA from total or poly(A)+ RNA. Used with PCR, this functionally tested system enables you to detect rare messages and generate enough material for cloning.

The SuperScript® III First-Strand Synthesis System:

Uses SuperScript® III RT for higher cDNA yields (Figure 1)

Includes all components needed for first-strand cDNA synthesis

Consists of optimized reagents for more robust performance

Contains a streamlined protocol

Detects template molecules from as little as 1.0 pg total

18080-051 SuperScript® III First-Strand 50 rxn

Synthesis System for RT-PCR

11668-027 Lipofectamine TM 2000 Reagent 0.75ml

11668-019 1.5ml

13778-150 Lipofectamine RNAiMax 1.5ml

13778-075 Lipofectamine RNAiMax 0.75ml

15596-026 TRlzol ®Reagent 100ml

15596-018 TRlzol ®Reagent 200ml

10296028 TRIZOL LS Reagent 200 ml（长方形） 200ml

10296010 TRIZOL LS Reagent 100ml（长方形） 100ml

11789020 BP CLONASE II ENZYME MIX 20 REACTIONS

11791020 LR CLONASE II ENZYME MIX 20 REACTIONS

12328040 AMV 100 REACTIONS

(CLON AMV FIRST STR SYNTH KIT)

28025-013 M-MLV Reverse Transcriptase 40,000U

17504-044 B-27（黄颜色） 10ml

12587-010 B-27（黄颜色）不含维生素A 10ml

15090-046 2.5% Trypsin (10X), no Phenol Red 100ml

15050-065 0.25% Trypsin(1X),Phenol Red 100ml

15050-057 500ml

15140-122 Pen Strep 100ml

15140-163 20X100ml

25200-056 0.25% Trypsin-EDTA(1X,Phenol Red) 100ml

25200-114 20X100ml

25300-054 0.05% Trypsin(1X),Phenol Red: 100ml

25300-120 20X100ml

15400-054 0.5% Trypsin(10X),: 100ml

25030-081 L-Glutamine 200mM 100ml

25030-164 20X100ml

12328019 CLONED AMV RT, 750U TRANSCRIPTASE

12328032 CLONED AMV FIRST STRA SYN KIT 25 REACTIONS

10966-018 Platinum® Taq DNA聚合酶 100 rxns

10966-026 250 rxns

10966-034 500 rxns

K210007 PURELINK PLASMID MAXI 25 25 REACTIONS

15224041 T4 DNA LIGASE HI CONC. 250 U

C737303 ONE SHOT STBL3 COMP E COLI ONE SHOT STBL3 20 RXN

12033015 ELECTROMAX DH10B T1 CELLS 0.5 ML (5 X 0.1 ML)

A10460 ONE SHOT CCDB SURVIVAL 2-T1R 10 REACTIONS

LC5925 SEEBLUE PLUS2 500UL

11708039 PLATINUM PFX DNA POLYMERASE 500 REACTIONS #95514- #52806

12344024 ACCUPRIME PFX DNA POLYMERASE 200 REACTIONS

12346086 ACCUPRIME TAQ HIFI 200 RXNS

11495017 PCRX ENHANCER SYSTEM 250 RXN 52395

450008 TOPO XL PCR CLONING KIT 10 RXN

MECOV50 METHYLCODE BISULFITE CONV KIT 50 REACTIONS

12935300 STEALTH RNAI NEG CTL MED GC 250 µl

2013 FLUORESCENT OLIGOMER, 20UM 250 UNITS

K159303 KIT,FAST TRACK 18 RXN

11917020 SS DBL STRND CDNA SYN KIT 50 RXNS

LC5677 MARK12 W RANGE MW STD,1ML 1 ML

18009019 CALF INTESTINAL ALK. PHOSPH. 1000 units

LC5688 HIMARK UNSTAINED HMW STD 250 UL

15622012 SUPERCOILED DNA LADDER 25 UG

S12000 SYPRO(R) RUBY PROTEIN GEL 1 L

R96025 ANTI-V5 ANTIBODY 50 UL

R93025 ANT-HIS(C-TERM) ANTIBODY 50 UL

717500 RABBIT ANTI-GST 100 UG

12579017 OPTIFECT 1 ML

14750100 BLOCKIT ALEXA FLUOR RED OLIGO 20 UM- 250UL

K494500 BLOCK IT U6 ENTRY VECTOR KIT 20 RXNS

15630080 HEPES 10ml

NP0060 NUPAGE MES BIS-TRIS BFR KIT COMBO

NP0321BOX NuPAGE® Novex 4-12% Bis-Tris Gel 1.0 mm, 10 Well 10 gels⁄box

12355036 PFX50 DNA POL., 500RXN 500 RXNS

A11180 CLONEMINER CDNA LIB KIT, VER2 5 RXN KIT

L150201 GENERACER SUPERSCRPT TA 5 CDNA- 50 PCR RXNS

11791020 LR CLONASE II ENZYME MIX 20 REACTIONS

10928-034 含有Platinum® Taq DNA聚合酶的SuperScriptTM II一步法RT-PCR系统 25 rxns

10928-042 100 rxns

11904-018 用于RT-PCR的SuperScriptTM IIDY链合成系统 50 rxns

18080-200 SuperScriptTM III CellsDirect cDNA合成试剂盒 25 rxns

18080-300 100 rxns

18080-400 SuperScriptTM IIIDY链合成SuperMix 50 rxns

18068-015 DNase I扩增级 100U(1U/ul)

48190-011 随机引物 9 units

K210005 PURELINK PLASMID MIDI 50 50 REACTIONS

S7563 SYBR® Green I核酸凝胶染料 500ul

S7567 1ml

S7564 SYBR® Green II RNA凝胶染料 500ul

S7568 1ml

11811031 GENETICIN 5G

10459014 DMRIE-C REAGENT 1ml

12309019 OPTI PRO SFM 1000ml

11514015 PLUS REAGENT 0.85 ML

12651014 FREESTYLE CHO EXPRESSION MEDIU 1000ML

12338018 FREESTYLE 293 EXPRESSION MED 1000ML

12347019 293FECTIN 1ml

16447100 FreeStyle MAX 1 ml

R25001 ZEOCIN 1g

15224-017 T4 DNA 连接酶 100U(1U/ul)

15224-025 500U(1U/ul)

15224-090 4x500U(1U/ul)

15224-041 250U(5U/ul)

18047-019 DNase I 20,000U

18068-015 DNase 扩增级 100U

18021-014 核糖核酸酶H 30U(2U/ul)

18021-071 4x30U(2U/ul)

18162-016 DNA聚合酶I/DNase I 250U

18005-017 T4 DNA 聚合酶 50U(5U/ul)

18005-025 250U(5U/ul)

25530-015 蛋白酶K 100mg

25530-031 1g

18374-058 5`RACE系统， 10 rxns

18373-019 3`RACE系统， 20 rxns

MPK10096 Neon™ Transfection System 100 µL Kit

K280020 ZERO BLUNT TOPO PCRCLON KIT 20 RXN

18012021 LARGE FRAGMENT 100 UN

18021014 RNASE H (E.COLI) 30 UN

4311816 AmpliTaq Gold® DNA Polymerase, 1000 units with Gold Buffer and MgCl2 solution 1000 un

18092015 CDNA SIZE FRAC COLUMNS 3 COLUMNS

450512 BLOCK-IT H1 RNAI VECTOR KIT 20 RXNS

10511012 1 kb DNA Extension Ladder 100 UG

K493600 POL II MIR RNAI GFP VECTOR 20 RXNS

10687010 Hygromycin B 20 ml

K158002 FastTrack MAG Maxi mRNA Isolation Kit    6 RXNS /盒

AB-4323161 SNaPshot Multiplex Kit 1000RXN

AM1560 mirVana miRNA Isolation Kit 40 purifications

AB-4366596 TaqMan® MicroRNA Reverse Transcription (RT) Kit 200RXN

AB-4324018 TaqMan® Universal PCR Master Mix 1*5ML

18374041 5' RACE REAGENT ASSY VER. 2.0 10 RXN

10787026 1 KB PLUS DNA LADDER 1000 UG

LC5602 MAGICMARK XP WESTERN STD 250 UL

11304029 PLATINUM TAQ HIGH FIDELITY 500 REACTIONS 95514- 52045

10966034 PLATINUM TAQ DNA POLYMERASE 600 reactions

K497500 VIRAPOWER PACKAGING MIX 195 UG X 3

R21001 BLASTICIDIN 50 MG

10814-010 UltraPure TM 糖原 100ul

15502-016 UltraPure TM 盐酸胍 500g

15535-016 UltraPure TM 异硫氰酸胍 500g

15577-018 UltraPure TM 异硫氰酸胍溶液 500ml

15593-031 UltraPure TM 酚: 氯仿：异戊醇 100ml

15525-017 UltraPure TM 十二烷基磺酸钠（SDS） 500g

31800-022 1640培养基 1640 Medium 10X1L原装

12800017 DMEM(高糖）含丙酮酸钠 10X1L原装

12100046 DMEM(高糖) DMEM(High Glucose) 10X1L原装

31600034 DMEM(低糖）DMEM(Low Glucose) 10X1L原装

41500034 MEM培养基 10X1L原装

31100035 M199 Medium  M199培养基 10X1L原装

21700075 F12培养基 10X1L原装

12400-024 D-MEM/F-12培养基 10X1L原装

12200-036 IMDM培养基 10X1L原装

12662029 MSC QUALIFIED FBS 500ML  msc细胞专用血清 500ML PLASTIC EZ HOLD

10438026 FBS, HI 500ML 热灭活胎牛血清 500ML PLASTIC EZ HOLD

10439024 FBS, ES CELL QUAL. USDA APPD. 500ML  干细胞专用血清 500ML PLASTIC EZ HOLD

10099141 FBS, AUSTRALIAN ORIGIN 500ML 澳洲优等胎牛血清 500ML

26400044 FBS, DIALYZED, USA ORIGIN 500ML透析胎牛美国 500ML PLASTIC EZ HOLD

26140079 FBS QUALIFIED, USA ORIGIN 500ML标准胎牛 500ML PLASTIC EZ HOLD

16141079 FBS, ES CELL QUAL, USA ORIGIN 500ML  干细胞专用胎牛 500ML PLASTIC EZ HOLD

16140071 FBS, HI 500ML 热灭活胎牛血清 500ML PLASTIC EZ HOLD

16000044 FBS, CERT, USA ORIGIN 500ML 特级北美 500ML PLASTIC EZ HOLD

C2027050 FBS, URUGUAYAN ORIGIN 乌拉圭血清 500ML

12657029 巴西胎牛 500ml

温馨提示：不可用于临床ZL。

内容物及储存（Contents and Storage）	The SuperScript® III First-Strand Synthesis System for RT-PCR is supplied with the components listed in Table 1. Store at -20°C. Guaranteed stable for 6 months when properly stored.
品Pai（Brand）	SuperScript®
反应形式（Reaction Format）	Separate Components
反转率酶（Reverse Transcriptase）	SuperScript® III
Downstream Application（Downstream Application）	RT-PCR (Endpoint),
最适反应温度（Optimal Reaction Temperature）	50° C
产品分类（Product Category）	分析试剂盒(Assay Kit)

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