磷酸化gp130抗体标记试剂磷酸化gp130抗体标记试剂,磷酸化gp130抗体标记价格,磷酸化gp130抗体标记标记试剂盒,各类抗体请来电详询!樊克生物将全心全意为您服务!
产品名:磷酸化gp130抗体
抗体英文名:Anti-phospho-gp130 (Ser782)
货号:FK-BJ5437
保存条件:Store at -20 °C
浓度:1mg/1ml
规格0.2ml/200μg
磷酸化gp130抗体标记试剂抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig, G
研究领域 肿瘤 细胞生物 免疫学 神经生物学 细胞粘附分子
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human MCSPG
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
磷酸化gp130抗体标记试剂抗体的应用:
可以用于做石蜡切片免疫组化,冰冻切片免疫组化,Elisa,WB,免疫荧光等实验。
(公司备有抗体及以下抗体做免疫组化与WB所需的二抗)
抗体应用的稀释:
1、Western Blotting (starting dilution 1:200, dilution range1:100-1:1000)
2、Immunoprecipitation [1-2 μg per 100-500 μg of total protein(1 ml ofcell lysate)]
3、Immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500)
4、ELISA (starting dilution 1:30, dilution range 1:30-1:3000)
5、Immunohistochemistry(including paraffin-embedded sections) (starting dilution 1:50
dilution range 1:50-1:500)
1 immunohistochemistry test principle: the principle of application of immunological antigen antibody reaction, namely the principle of combination of antigen and antibody specificity, chemically chromogenic agent (fluorescein labeled antibody, enzyme, metal ion, isotope) color to determine the tissue cells antigen (peptide and protein), study the qualitative and the quantity and the location, the immunohistochemical technique, called.
Immunohistochemical technique according to the types of markers can be divided into immunofluorescence, immunoperoxidase assay, immuno ferritin method, colloidal gold method and radioimmunoassay autoradiography method etc
Experiment 2: the basic principle of immune fluorescence immunological reaction is the antigen antibody reaction. The antigen antibody reaction is highly specific, so when the antigen antibody reaction, just know that one of the factors, we can find out another factor. Immunofluorescence technique that will not affect the activity of antigen and antibody fluorescent dye labeled antibody (or antigen), and its corresponding antigen (or antibody) combined, showing a specific fluorescence under fluorescent microscope.
注:以上信息仅供参考,具体产品介绍请来电或在线方式咨询。
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FK-BJ5437,磷酸化gp130抗体标记试剂,规格:0.2ml/200μg,浓度