StemRNA™-NM Reprogramming Kit

Stemgent’s latest evolution in RNA reprogramming combines non-modified RNA and microRNA technology to provide a kit for stem cell researchers with a new level of versatility, simplicity and time savings, enabling cellular reprogramming of human fibroblasts, and cells from blood and now urine for difficult to reprogram patient samples.
 Key Benefits

• Flexible technology generates high-quality human iPS cell lines from multiple target cell types
  Out-of-the-box reprogramming of cells from skin (fibroblasts), blood (endothelial progenitor cells; EPCs) and urine (urine-derived epithelial cells; UDCs)
• High efficiency, non-integrating reprogramming
  StemRNA-NM requires a few as four additional reagents. Double stranded microRNAs enhance reprogramming efficiency, prividing iPS cells with high efficiency (up to 4% from fibroblasts, up to 3% from EPCs, up to 0.5% from UDCs).
• Time-saving protocol delivers faster results facilitating higher throughput
  Colonies ready to pick in 10-14 days from fibroblasts and 12-16 days from EPCs or UDCs.  No screening needed, saving an additional 2-10 weeks (3-10+ passages) needed for vector clearance with other technologies.
•  

	StemRNA-NM (00-0076)
Feature	Fibroblasts	Urine (UDCs)	Blood (EPCs)
No. wells per kit	9	3	3
No Transfections required	4	6-8	6-8
Days to primary iPS cell colonies	10-14	12-14	12-14
Reprogramming efficiency	2-+4%	0.1-0.5%	0.4-3%
Screening Required	No	No	No
Xeno-compatible protocol	Yes	No	No
GMP-compatible RNA manufacturing protocol	Yes	Yes	Yes

 

Size

1 kit

Kit Contents

• OKSMNL NM-RNA (Part No. 05-0040), 30 µg, 1 vial
• EKB NM-RNA (Part No. 05-0041), 22 µg, 1 vial
• NM-microRNAs (Part No. 05-0042), 15 µg, 1 vial

Storage and Stability

1. Store all three kit components at or below -70 °C.
2. Kit components are stable for a minimum of 3 months from date of receipt when stored as directed.

Quality Control

The individual mRNAs are tested for size and integrity. The StemRNA™-SR Reprogramming Kit is functionally validated for successful RNA-based reprogramming of adult fibroblasts and human umbilical vein endothelial cells (HUVECs). Complete reprogramming of iPS cell colonies is confirmed by expression of pluripotency markers and appropriate colony morphology. All components of the kit are sterile and have tested negative for mycoplasma.

Limited Use Licenses

This product is intended for research use only. This product uses technology exclusively licensed from Biontech AG, as described in WO 2009/077134. This product may also be covered by one or more of WO 2007/036366, WO 2008/157688, WO 2012/072269, and WO 2014/072061, licensed from Biontech AG.

Limited Use License iPS Academia Japan

Recommended Usage

For use with associated StemRNA-NM protocols for generation of iPS cells.