Reactivity | Mouse, Human, Rat |
Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA). |
Preparation | Streptavidin was conjugated with Brilliant Violet 650™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 650™. |
Concentration | 0.1 |
Concentration Units | mg/ml |
Recommended Usage | Each lot of this Streptavidin-Brilliant Violet 650™ is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, we recommend using ≤0.125 µg in 100 µl staining volume per million cells. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd. |
Applications | FC, ICFC - Quality tested |
Description | Streptavidin binds to biotin with high affinity. Streptavidin-Brilliant Violet 650™ is useful for detecting biotinylated antibodies. The excitation of Brilliant Violet 650™ by 405 nm laser light induces a fluorescence maximum emission of 645 nm. |
Storage | The streptavidin solution should be stored undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze. |