Brilliant Violet 421™ Armenian Hamster IgG Isotype Ctrl_详细资料

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克隆性：多克隆保存条件：蓝冰规格： 500 ul

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Brilliant Violet 421™ Armenian Hamster IgG Isotype Ctrl参数介绍>>>>

Clone HTK888
Isotype Armenian Hamster IgG
Immunogen Trinitrophenol + KLH
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Applications
FC, ICFC - Quality tested
Application Notes
The HTK888 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, functional assay, immunofluorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 400916) as negative control. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400940) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References
1. Lesley R, et al. 2006. P. Natl. Acad. Sci. USA 103:10717.
2. Yu R, et al. 2006. Obesity 14:1353.
3. Yang JH, et al.2005. Rheumatology(Oxford). 44:1245. PubMed
3. Mina-Osorio P, et al. 2008. J. Leukocyte Biol. 84:448. PubMed
4. Shen H, et al. 2009. J. Am Soc Nephrol. 20:1032. PubMed
"
Description
This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Storage The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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Brilliant Violet 421™ Armenian Hamster IgG Isotype Ctrl

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Clone	HTK888
Isotype	Armenian Hamster IgG
Immunogen	Trinitrophenol + KLH
Formulation	Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation	The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Recommended Usage	Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Applications	FC, ICFC - Quality tested
Application Notes	The HTK888 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, functional assay, immunofluorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 400916) as negative control. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400940) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References	1. Lesley R, et al. 2006. P. Natl. Acad. Sci. USA 103:10717. 2. Yu R, et al. 2006. Obesity 14:1353. 3. Yang JH, et al.2005. Rheumatology(Oxford). 44:1245. PubMed 3. Mina-Osorio P, et al. 2008. J. Leukocyte Biol. 84:448. PubMed 4. Shen H, et al. 2009. J. Am Soc Nephrol. 20:1032. PubMed "
Description	This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Storage	The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.