Brilliant Violet 421™ anti-human CD279 (PD-1)_详细资料

产品信息

克隆性：多克隆保存条件：蓝冰规格： 100 tests

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Brilliant Violet 421™ anti-human CD279 (PD-1)参数介绍>>>>

Clone EH12.2H7
Isotype Mouse IgG1, κ
Reactivity Human, Cross-Reactivity: Chimpanzee, Common Marmoset, Rhesus¹², Squirrel Monkey, Cynomolgus
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Applications
FC - Quality tested
Application Notes
Additional reported applications (for the relevant formats) include: blocking of ligand binding^1-3. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 329911 and 329912). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 329926) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
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Application References
1. Dorfman DM, et al. 2006 Am. J. Surg. Pathol. 30:802. (FA)
2. Radziewicz H, et al. 2007. J. Virol. 81:2545. (FA)
3. Velu V, et al. 2007. J. Virol. 81:5819.
4. Zahn RC, et al. 2008. J. Virol. 82:11577. PubMed
5. Chang WS, et al. 2008. J. Immunol. 181:6707. (FC) PubMed
6. Nakamoto N, et al. 2009. PLoS Pathog. 5:e1000313. (FA)
7. Jones RB, et al. 2009. J. Virol. 83:8722. (FC) PubMed
8. Vojnov L, et al. 2010. J. Virol. 84:753. (FC) PubMed
9. Radziewicz H, et al. 2010. J. Immunol. 184:2410. (FC) PubMed
10. Monteriro P, et al. 2011. J. Immunol. 186:4618. PubMed
11. Conrad J, et al. 2011. J. Immunol. 186:6871. PubMed
12. Salisch NC, et al. 2010. J. Immunol. 184:476.
"
Description
The program death 1 (PD-1) receptor CD279 is a 55 kD member of the immunoglobulin superfamily. CD279/PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279/PD-1 is expressed predominantly on activated T cells, B cells and myeloid cells. PD-L1 (B7-H1) and PD-L2 (B7-DC) are ligands of CD279/PD-1 and are members of the B7 gene family. Evidence reported to date suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279:PD-ligands results in inhibition of T cell proliferation and cytokine secretion.
Storage The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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Clone	EH12.2H7
Isotype	Mouse IgG1, κ
Reactivity	Human, Cross-Reactivity: Chimpanzee, Common Marmoset, Rhesus¹², Squirrel Monkey, Cynomolgus
Formulation	Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation	The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Recommended Usage	Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Applications	FC - Quality tested
Application Notes	Additional reported applications (for the relevant formats) include: blocking of ligand binding^1-3. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 329911 and 329912). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 329926) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg). "
Application References	1. Dorfman DM, et al. 2006 Am. J. Surg. Pathol. 30:802. (FA) 2. Radziewicz H, et al. 2007. J. Virol. 81:2545. (FA) 3. Velu V, et al. 2007. J. Virol. 81:5819. 4. Zahn RC, et al. 2008. J. Virol. 82:11577. PubMed 5. Chang WS, et al. 2008. J. Immunol. 181:6707. (FC) PubMed 6. Nakamoto N, et al. 2009. PLoS Pathog. 5:e1000313. (FA) 7. Jones RB, et al. 2009. J. Virol. 83:8722. (FC) PubMed 8. Vojnov L, et al. 2010. J. Virol. 84:753. (FC) PubMed 9. Radziewicz H, et al. 2010. J. Immunol. 184:2410. (FC) PubMed 10. Monteriro P, et al. 2011. J. Immunol. 186:4618. PubMed 11. Conrad J, et al. 2011. J. Immunol. 186:6871. PubMed 12. Salisch NC, et al. 2010. J. Immunol. 184:476. "
Description	The program death 1 (PD-1) receptor CD279 is a 55 kD member of the immunoglobulin superfamily. CD279/PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279/PD-1 is expressed predominantly on activated T cells, B cells and myeloid cells. PD-L1 (B7-H1) and PD-L2 (B7-DC) are ligands of CD279/PD-1 and are members of the B7 gene family. Evidence reported to date suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279:PD-ligands results in inhibition of T cell proliferation and cytokine secretion.
Storage	The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.