Biotin anti-human/mouse CD49f_详细资料

产品信息

克隆性：多克隆保存条件：蓝冰规格： 100 ug

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Biotin anti-human/mouse CD49f参数介绍>>>>

Clone GoH3
Isotype Rat IgG2a, κ
Reactivity Human, Mouse, Cross-Reactivity: Baboon, Chimpanzee, Capuchin Monkey, Cynomolgus, Rhesus, Horse (Equine), Cattle (Bovine, Cow), Sheep (Ovine), Swine (Pig, Porcine), Dog (Canine), Cat (Feline), Rabbit (Lapine)
Immunogen Mouse mammary tumor cells
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration 0.5mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Applications
FC - Quality tested
Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation^1,5, in vitro and in vivo blocking of cell binding to laminin and blocking the function of integrin α₆^1,4, and immunohistochemistry of acetone-fixed frozen sections^2,3,5. The GoH3 antibody has been reported to block laminin binding in vitro and to block integrin α₆ function in vivo.
Application References
1. Georas SN, et al. 1993. Blood 82:2872. (IP, Block)
2. Honda T, et al. 1995. J. Clin. Endocrinol. Metab. 80:2899. (IHC)
3. Sonnenberg A, et al. 1986. J. Histochem. Cytochem. 34:1037. (IHC)
4. Nakamura K, et al. 1997 Biochem. Biophys. Res. Commun. 235:524. (Block)
5. Sonnenberg A, et al. 1987 J. Biol. Chem. 262:10376. (IP, IHC)
6. Deregibus MC, et al. 2007. Blood doi:10.1182/blood-2007-03-078709.
7. Horwitz KB, et al. 2008. Proc Natl Acad Sci USA. 105:5774. PubMed
8. Nardella C, et al. 2009. Sci Signal. 2:55. PubMed
9. Xu T, et al. 2010. Mol Cancer Ther. 9:438. PubMed
10. Stepp MA, et al. 2007. J Cell Sci. 120:2851. PubMed
11. Jo M, et al. 2010. Cancer Res. 70:8948. PubMed
12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
13. Grange C, et al. 2011. Cancer Res. 71:5346. PubMed
14. Lai KP, et al. 2012. Mol Endocrinol. 26:52. PubMed
"
Description
CD49f is a 120 kD integrin family member also known as VLA-6 α chain and α₆ integrin subunit. CD49f associates with either integrin β₁ (CD29) or integrin β₄ (CD104) to form receptors (VLA-6 or α₆β₄ complex) for laminin and kalinin. CD49f is expressed on platelets, monocytes, T cells, placental trophoblasts, epithelial and endothelial cells. CD49f is involved in adhesion and can act as a co-stimulatory molecule for T cell activation and proliferation.
Storage The antibody solution should be stored undiluted at 4°C. Do not freeze.

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Clone	GoH3
Isotype	Rat IgG2a, κ
Reactivity	Human, Mouse, Cross-Reactivity: Baboon, Chimpanzee, Capuchin Monkey, Cynomolgus, Rhesus, Horse (Equine), Cattle (Bovine, Cow), Sheep (Ovine), Swine (Pig, Porcine), Dog (Canine), Cat (Feline), Rabbit (Lapine)
Immunogen	Mouse mammary tumor cells
Formulation	Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation	The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration	0.5mg/ml
Recommended Usage	Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Applications	FC - Quality tested
Application Notes	Additional reported applications (for the relevant formats) include: immunoprecipitation^1,5, in vitro and in vivo blocking of cell binding to laminin and blocking the function of integrin α₆^1,4, and immunohistochemistry of acetone-fixed frozen sections^2,3,5. The GoH3 antibody has been reported to block laminin binding in vitro and to block integrin α₆ function in vivo.
Application References	1. Georas SN, et al. 1993. Blood 82:2872. (IP, Block) 2. Honda T, et al. 1995. J. Clin. Endocrinol. Metab. 80:2899. (IHC) 3. Sonnenberg A, et al. 1986. J. Histochem. Cytochem. 34:1037. (IHC) 4. Nakamura K, et al. 1997 Biochem. Biophys. Res. Commun. 235:524. (Block) 5. Sonnenberg A, et al. 1987 J. Biol. Chem. 262:10376. (IP, IHC) 6. Deregibus MC, et al. 2007. Blood doi:10.1182/blood-2007-03-078709. 7. Horwitz KB, et al. 2008. Proc Natl Acad Sci USA. 105:5774. PubMed 8. Nardella C, et al. 2009. Sci Signal. 2:55. PubMed 9. Xu T, et al. 2010. Mol Cancer Ther. 9:438. PubMed 10. Stepp MA, et al. 2007. J Cell Sci. 120:2851. PubMed 11. Jo M, et al. 2010. Cancer Res. 70:8948. PubMed 12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) 13. Grange C, et al. 2011. Cancer Res. 71:5346. PubMed 14. Lai KP, et al. 2012. Mol Endocrinol. 26:52. PubMed "
Description	CD49f is a 120 kD integrin family member also known as VLA-6 α chain and α₆ integrin subunit. CD49f associates with either integrin β₁ (CD29) or integrin β₄ (CD104) to form receptors (VLA-6 or α₆β₄ complex) for laminin and kalinin. CD49f is expressed on platelets, monocytes, T cells, placental trophoblasts, epithelial and endothelial cells. CD49f is involved in adhesion and can act as a co-stimulatory molecule for T cell activation and proliferation.
Storage	The antibody solution should be stored undiluted at 4°C. Do not freeze.