FITC anti-mouse Lineage Cocktail with Isotype Ctrl_详细资料

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克隆性：多克隆保存条件：蓝冰规格： 25 tests

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FITC anti-mouse Lineage Cocktail with Isotype Ctrl参数介绍>>>>

Clone 145-2c11/RB6-8C5/RA3-6B2/Ter-119/M1/70
Isotype Rat IgG2b/Rat IgG2a/Hamster IgG
Reactivity Mouse
Formulation This two-reagent set contains FITC conjugated antibodies and isotype controls in optimal concentration for flow cytometric analysis.
Preparation The antibodies are purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution are free of unconjugated FITC.
Recommended Usage
Each lot of these antibodies is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Applications
FC - Quality tested
Application References
1. Morris SJ, et al. 1997. Development. 124(10):1929-1939
2. Okada S, et al. 1992. Blood180 (12):3044-3050
3. Spangrude GJ, et al. 1988. Science 241 (4861):58-62
4. Spangrude GJ, et al. 1990. Exp Hematol. 18 (8):920-926
5. Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed
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Description
The mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, NK cells, and erythrocytes. The FITC Mouse Lineage Antibody Cocktail is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include anti-mouse CD3e, clone 145-2C11; anti-mouse Ly-6G/Ly-6C, clone RB6-8C5; anti-mouse CD11b, clone M1/70; anti-mouse CD45R/B220, clone RA3-6B2; anti-mouse TER-119/Erythroid cells, clone Ter-119. FITC Mouse Lineage Isotype Control Cocktail contains equivalent concentrations of isotype-matched negative control immunoglobulin.

Materials provided:
FITC anti-mCD3/ FITC anti-mGr-1/ FITC anti-mCD11b/ FITC anti-mCD45R(B220)/ FITC anti-mTer-119, 25 or 100 tests
FITC Armenian hamster IgG/ FITC Rat IgG2b/ FITC Rat IgG2a isotype controls, 25 or 100 tests
Materials not provided:
Cell Staining Buffer (Cat. No. 420201)
Storage The antibody solutions should be stored undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.

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FITC anti-mouse Lineage Cocktail with Isotype Ctrl

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Clone	145-2c11/RB6-8C5/RA3-6B2/Ter-119/M1/70
Isotype	Rat IgG2b/Rat IgG2a/Hamster IgG
Reactivity	Mouse
Formulation	This two-reagent set contains FITC conjugated antibodies and isotype controls in optimal concentration for flow cytometric analysis.
Preparation	The antibodies are purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution are free of unconjugated FITC.
Recommended Usage	Each lot of these antibodies is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Applications	FC - Quality tested
Application References	1. Morris SJ, et al. 1997. Development. 124(10):1929-1939 2. Okada S, et al. 1992. Blood180 (12):3044-3050 3. Spangrude GJ, et al. 1988. Science 241 (4861):58-62 4. Spangrude GJ, et al. 1990. Exp Hematol. 18 (8):920-926 5. Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed "
Description	The mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, NK cells, and erythrocytes. The FITC Mouse Lineage Antibody Cocktail is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include anti-mouse CD3e, clone 145-2C11; anti-mouse Ly-6G/Ly-6C, clone RB6-8C5; anti-mouse CD11b, clone M1/70; anti-mouse CD45R/B220, clone RA3-6B2; anti-mouse TER-119/Erythroid cells, clone Ter-119. FITC Mouse Lineage Isotype Control Cocktail contains equivalent concentrations of isotype-matched negative control immunoglobulin. Materials provided: FITC anti-mCD3/ FITC anti-mGr-1/ FITC anti-mCD11b/ FITC anti-mCD45R(B220)/ FITC anti-mTer-119, 25 or 100 tests FITC Armenian hamster IgG/ FITC Rat IgG2b/ FITC Rat IgG2a isotype controls, 25 or 100 tests Materials not provided: Cell Staining Buffer (Cat. No. 420201)
Storage	The antibody solutions should be stored undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.