Clone | 1B11 |
Isotype | Rat IgG2a, κ |
Reactivity | Mouse |
Immunogen | Mouse WEHI 274.3 myeloid tumor cells |
Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
Preparation | The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody. |
Concentration | 0.2mg/ml |
Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cy5.5 has a maximum absorption of 482 nm and 564 nm and a maximum emission of 690 nm. |
Applications | FC - Quality tested |
Application Notes | Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, and immunohistochemistry of acetone-fixed frozen sections. |
Application References | 1. Jones AT, et al. 1994. J. Immunol. 153:3426. (IP, WB) 2. Carlow DA, et al. 1999. J. Immunol. 163:1441. (IP, WB) 3. Onami TM, et al. 2002. J. Immunol. 168:6022. 4. van der Most RG, et al. 2003. Intl. Immunol. 15:119. 5. Chu VT, et al. 2007. J. Immunol. 179:5947. 6. Lang A, et al. 2008. J. Immunol. 180:4848. PubMed 7. Gibbert K, et al. 2010. J. Immunol. 185:6179. PubMed " |
Description | CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and gp115, is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. Due to variable glycosylation and sialylation, two isoforms of CD43 have been identified. The 115 kD glycoform of CD43 is expressed on most hematopietic cells including T lymphocytes, NK cells, monocytes, granulocytes, platelets, and CD5+ B cells. It does not present on resting B cells and erythrocytes. While the 130 kD glycoform is thought to be activation-associated form primarily expressed on myeloid cells, pre-B cells, and activated T cells. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion, as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors. The 1B11 antibody reacts with the activation-associated glycoform of CD43. The epitope recognized by 1B11 is also shared with desialylated CD45RB. This antibody is useful for differentiation of effector CD8 T cells and memory T cells. |
Storage | The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |