PerCP/Cy5.5 anti-mouse TER-119/Erythroid Cells_详细资料

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克隆性：多克隆保存条件：蓝冰规格： 100 ug

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PerCP/Cy5.5 anti-mouse TER-119/Erythroid Cells参数介绍>>>>

Clone TER-119
Isotype Rat IgG2b, κ
Reactivity Mouse
Immunogen Day-14 fetal liver cells from a C57BL/6 mouse
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration 0.2mg/ml
Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cy5.5 has a maximum absorption of 482 nm and 564 nm and a maximum emission of 690 nm.
Applications
FC - Quality tested
Application Notes
The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation¹, Western blotting¹, complement-mediated cytotoxicity³, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 116214).
Application References
1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
2. Vannucchi AM, et al. 2000. Blood 95:2559.
3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
4. Grisendi S, et al. 2005. Nature 437:147. (FC)
5. Bourdeau A, et al. 2007. Blood 109:4220.
6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
7. Heuser M, et al. 2007. Blood 110:1639. (FC)
Description
The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on BFU-E or CFU-E.
Storage The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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Clone	TER-119
Isotype	Rat IgG2b, κ
Reactivity	Mouse
Immunogen	Day-14 fetal liver cells from a C57BL/6 mouse
Formulation	Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation	The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration	0.2mg/ml
Recommended Usage	Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cy5.5 has a maximum absorption of 482 nm and 564 nm and a maximum emission of 690 nm.
Applications	FC - Quality tested
Application Notes	The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation¹, Western blotting¹, complement-mediated cytotoxicity³, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 116214).
Application References	1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB) 2. Vannucchi AM, et al. 2000. Blood 95:2559. 3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD) 4. Grisendi S, et al. 2005. Nature 437:147. (FC) 5. Bourdeau A, et al. 2007. Blood 109:4220. 6. Chappaz S, et al. 2007. Blood 110:3862. (FC) 7. Heuser M, et al. 2007. Blood 110:1639. (FC)
Description	The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on BFU-E or CFU-E.
Storage	The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.