PerCP anti-mouse CD45.2_详细资料

产品信息

克隆性：多克隆保存条件：蓝冰规格： 100 ug

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PerCP anti-mouse CD45.2参数介绍>>>>

Clone 104
Isotype Mouse (SJL) IgG2a, κ
Reactivity Mouse
Immunogen B10.S mouse thymocytes and splenocytes
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions. The solution is free of unconjugated PerCP and unconjugated antibody.
Concentration 0.2mg/ml
Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.
Applications
FC - Quality tested
Application Notes
The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation⁴, in vivo and in vitro blocking of B cell responses^1,2, and immunohistochemical staining of acetone-fixed frozen sections³.
Application References
1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
6. Pascal V, et al. 2007. J. Immunol. 179:1751.
7. Burman AC, et al. 2007. Blood 110:1064.
8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
9. Phan TG, et al. 2007. Nature Immunol. 8:992.
10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
"
Description
CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
Storage The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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Clone	104
Isotype	Mouse (SJL) IgG2a, κ
Reactivity	Mouse
Immunogen	B10.S mouse thymocytes and splenocytes
Formulation	Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation	The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions. The solution is free of unconjugated PerCP and unconjugated antibody.
Concentration	0.2mg/ml
Recommended Usage	Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.
Applications	FC - Quality tested
Application Notes	The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation⁴, in vivo and in vitro blocking of B cell responses^1,2, and immunohistochemical staining of acetone-fixed frozen sections³.
Application References	1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block) 2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block) 3. Suzuki K, et al. 2000. Immunity 13:691. (IHC) 4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP) 5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837. 6. Pascal V, et al. 2007. J. Immunol. 179:1751. 7. Burman AC, et al. 2007. Blood 110:1064. 8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187. 9. Phan TG, et al. 2007. Nature Immunol. 8:992. 10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed "
Description	CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
Storage	The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.