Purified anti-mouse CD107b (Mac-3)_详细资料

产品信息

克隆性：多克隆保存条件：蓝冰规格： 500 ug

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Purified anti-mouse CD107b (Mac-3)参数介绍>>>>

Clone M3/84
Isotype Rat IgG1, κ
Reactivity Mouse
Immunogen Membrane glycoproteins from C57BL/6 mouse peritoneal exudate cells
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography.
Concentration 0.5mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Applications
FC - Quality tested
IP, IHC - Reported in the literature
Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation^1-4 and immunohistochemical staining^5,6 of acetone-fixed frozen sections.
Application References
1. Springer TA. 1981. J. Biol. Chem. 256:3833. (IP)
2. Ho MK, et al. 1983. J. Biol. Chem. 258:636. (IP)
3. Chen JW, et al. 1985. J. Cell Biol. 101:85. (IP)
4. Ralph P, et al. 1983. J. Immunol. 130:108. (IP)
5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
6. Kano M, et al. 1998. Transplantation 65:837. (IHC)
7. Terrazas LI,et al.2005. Int J Parasitol. 35:1349. PubMed
8. Hayashida A, et al. 2011. J. Biol Chem. 286:3288. PubMed
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Description
Mac-3 is a 110 kD type I membrane glycoprotein, also known as CD107b and LAMP-2. It is expressed on lysosomal membranes and the plasma membrane of macrophages and some myeloid cell lines. In the bone marrow, few cells display Mac-3 antigen on the surface, but a large proportion express Mac-3 in the cytoplasm. CD107b has been identified as a ligand for galaptin, an S-type lectin present in the extracellular matrix. Mac-3/CD107b is upregulated in some tumors and increased expression has been correlated with enhanced metastatic potential.
Storage The antibody solution should be stored undiluted at 4°C.

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Clone	M3/84
Isotype	Rat IgG1, κ
Reactivity	Mouse
Immunogen	Membrane glycoproteins from C57BL/6 mouse peritoneal exudate cells
Formulation	Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation	The antibody was purified by affinity chromatography.
Concentration	0.5mg/ml
Recommended Usage	Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Applications	FC - Quality tested IP, IHC - Reported in the literature
Application Notes	Additional reported applications (for the relevant formats) include: immunoprecipitation^1-4 and immunohistochemical staining^5,6 of acetone-fixed frozen sections.
Application References	1. Springer TA. 1981. J. Biol. Chem. 256:3833. (IP) 2. Ho MK, et al. 1983. J. Biol. Chem. 258:636. (IP) 3. Chen JW, et al. 1985. J. Cell Biol. 101:85. (IP) 4. Ralph P, et al. 1983. J. Immunol. 130:108. (IP) 5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC) 6. Kano M, et al. 1998. Transplantation 65:837. (IHC) 7. Terrazas LI,et al.2005. Int J Parasitol. 35:1349. PubMed 8. Hayashida A, et al. 2011. J. Biol Chem. 286:3288. PubMed "
Description	Mac-3 is a 110 kD type I membrane glycoprotein, also known as CD107b and LAMP-2. It is expressed on lysosomal membranes and the plasma membrane of macrophages and some myeloid cell lines. In the bone marrow, few cells display Mac-3 antigen on the surface, but a large proportion express Mac-3 in the cytoplasm. CD107b has been identified as a ligand for galaptin, an S-type lectin present in the extracellular matrix. Mac-3/CD107b is upregulated in some tumors and increased expression has been correlated with enhanced metastatic potential.
Storage	The antibody solution should be stored undiluted at 4°C.