LEAF™ Purified anti-mouse CD273 (B7-DC, PD-L2)_详细资料

产品信息

克隆性：多克隆保存条件：蓝冰规格： 500 ug

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LEAF™ Purified anti-mouse CD273 (B7-DC, PD-L2)参数介绍>>>>

Clone TY25
Isotype Rat IgG2a, κ
Reactivity Mouse
Immunogen Mouse B7-DC transfected cell line
Formulation 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration 1mg/ml
Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Applications
FC - Quality tested

WB, IP, Block, IHC - Reported in the literature
Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation¹, Western blotting^1,6, blocking^2,4,5 of PD-1 mediated interactions, and immunohistochemistry of acetone-fixed frozen sections³. The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 107208).
"
Application References
1. Yamazaki T, et al. 2002. J. Immunol. 169:5538. (FC IP WB)
2. Ansari MJI, et al. 2003. J. Exp. Med. 198:63. (Block)
3. Salama AD, et al. 2003. J. Exp. Med. 198:71. (IHC)
4. Matsumoto K, et al. 2004. J. Immunol. 172:2530. (FC Block)
5. Yamazaki T, et al. 2005. J. Immunol. 175:1586. (Block)
6. Meng Q, et al. 2006. Invest Opthalmol Vis Sci. 47:444. (WB) PubMed
"
Description
B7-DC is also called programmed death ligand 2 (PDL2). It has recently been clustered as CD273. This ligand is a 42 kD member of the immunoglobulin receptor superfamily expressed on a subset of dendritic cells, liver and a small subset of macrophages as well as a few transformed cell lines. CD273 has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engaging the PD-1 receptor. CD273 has also been reported to bind to an alternative receptor and to mediate T cell activation through such non-PD1 mediated interactions. The TY25 antibody has been reported to be useful for blocking PD-1 mediated interactions.
"
Storage The antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.

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LEAF™ Purified anti-mouse CD273 (B7-DC, PD-L2)

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Clone	TY25
Isotype	Rat IgG2a, κ
Reactivity	Mouse
Immunogen	Mouse B7-DC transfected cell line
Formulation	0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation	The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration	1mg/ml
Recommended Usage	Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Applications	FC - Quality tested WB, IP, Block, IHC - Reported in the literature
Application Notes	Additional reported applications (for the relevant formats) include: immunoprecipitation¹, Western blotting^1,6, blocking^2,4,5 of PD-1 mediated interactions, and immunohistochemistry of acetone-fixed frozen sections³. The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 107208). "
Application References	1. Yamazaki T, et al. 2002. J. Immunol. 169:5538. (FC IP WB) 2. Ansari MJI, et al. 2003. J. Exp. Med. 198:63. (Block) 3. Salama AD, et al. 2003. J. Exp. Med. 198:71. (IHC) 4. Matsumoto K, et al. 2004. J. Immunol. 172:2530. (FC Block) 5. Yamazaki T, et al. 2005. J. Immunol. 175:1586. (Block) 6. Meng Q, et al. 2006. Invest Opthalmol Vis Sci. 47:444. (WB) PubMed "
Description	B7-DC is also called programmed death ligand 2 (PDL2). It has recently been clustered as CD273. This ligand is a 42 kD member of the immunoglobulin receptor superfamily expressed on a subset of dendritic cells, liver and a small subset of macrophages as well as a few transformed cell lines. CD273 has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engaging the PD-1 receptor. CD273 has also been reported to bind to an alternative receptor and to mediate T cell activation through such non-PD1 mediated interactions. The TY25 antibody has been reported to be useful for blocking PD-1 mediated interactions. "
Storage	The antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.