PerCP anti-mouse/human CD44_详细资料

产品信息

克隆性：多克隆保存条件：蓝冰规格： 100 ug

上海科敏生物经销国内外知名品Pai试剂抗体，折扣低，货期快，欢迎新老客户咨询订购！
PerCP anti-mouse/human CD44参数介绍>>>>

Clone IM7
Isotype Rat IgG2b, κ
Reactivity Mouse, Human, Cross-Reactivity: Baboon, Chimpanzee, Cynomolgus, Rhesus, Squirrel Monkey, Horse (Equine), Cattle (Bovine, Cow), Swine (Pig, Porcine), Dog (Canine), Cat (Feline)
Immunogen Dexamethasone-induced myeloid leukemia M1 cells
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography and conjugated with PerCP under optimal conditions. The solution is free of unconjugated PerCP and unconjugated antibody.
Concentration 0.2mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.
Applications
FC - Quality tested

Application Notes
Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD44^17,18. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections^6,7, complement-mediated cytotoxicity¹, immunoprecipitation^1,3, and in vivo inhibition of DTH^4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 103014). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 103046) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References
1. Trowbridge IS, et al. 1982. Immunogenetics 15:299. (ICFC, IP, CMCD)
2. Katoh S, et al. 1994. J. Immunol. 153:3440. (ELISA)
3. Budd RC, et al. 1987. J. Immunol. 138:3120. (IP)
4. Camp RL, et al. 1993. J. Exp. Med. 178:497. (Block)
5. Weiss JM, et al. 1997. J. Cell Biol. 137:1137. (Block)
6. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC) PubMed
7. Cuff CA, et al. 2001. J. Clin. Invest. 108:1031. (IHC)
8. Lee JW, et al. 2006. Nature Immunol. 8:181.
9. Zhang N, et al. 2005. J. Immunol. 174:6967. PubMed
10. Huabiao C, et al. 2005. J. Immunol. 175:591. PubMed
11. Gui J, et al. 2007. Int. Immunol. 19:1201. PubMed
12. Wang XY, et al. 2008. Blood 111:2436. PubMed
13. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
14. Yamazaki J, et al. 2009. Blood PubMed
15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
16. Chen YW, et al. 2010. Mol. Cancer Ther. 9:2879. PubMed
17. Zheng Z, et al. 1995. J. Cell. Biol. 130:485.
18. Wiranowska M, et al. 2010. Int. J. Cancer 127:532.
"
Description
CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.
Storage The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

品Pai介绍：Biolegend公司自2002年成立以来，一直致力于为生物医药研究领域提供YL的，高质量的流式抗体、试剂盒及其它相关产品。公司聚集了一批来自PharMingen的专业杰出人士，建立了一支雄厚技术背景的专业队伍，并通过其自身的先天优势，以及与世界知名实验室和研究所的良好合作关系，以最合理的价格为广大客户提供ZY的免疫学和细胞生物学产品，并不断地开发新产品，以满足飞速发展的生命科学研究的需要。短短几年时间，Biolegend公司得到了飞速发展，已跻身ZX进的抗体公司行列，并在2009年成为美国哈佛大家的抗体供应商！Biolegend公司的试剂主要包括细胞免疫、细胞因子、趋化因子、粘附分子等及与癌症研究、T 调节细胞研究、干细胞研究、先天免疫研究、细胞周期分析、凋亡研究及特定修饰类抗体等领域相关的试剂，用于流式、ELISA、免疫沉淀、WB、免疫荧光、免疫组化、及体内体外功能学试验。其中，Biolegend公司的流式抗体以其具有较宽范围的荧光选择及较高的性价比，为广大科研工作者所接受！同时，公司配备了一支经验丰富的技术服务队伍，为客户提供WM的配色方案及快速的售后处理服务！

您可能感兴趣的产品

Clone	IM7
Isotype	Rat IgG2b, κ
Reactivity	Mouse, Human, Cross-Reactivity: Baboon, Chimpanzee, Cynomolgus, Rhesus, Squirrel Monkey, Horse (Equine), Cattle (Bovine, Cow), Swine (Pig, Porcine), Dog (Canine), Cat (Feline)
Immunogen	Dexamethasone-induced myeloid leukemia M1 cells
Formulation	Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation	The antibody was purified by affinity chromatography and conjugated with PerCP under optimal conditions. The solution is free of unconjugated PerCP and unconjugated antibody.
Concentration	0.2mg/ml
Recommended Usage	Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.
Applications	FC - Quality tested
Application Notes	Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD44^17,18. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections^6,7, complement-mediated cytotoxicity¹, immunoprecipitation^1,3, and in vivo inhibition of DTH^4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 103014). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 103046) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References	1. Trowbridge IS, et al. 1982. Immunogenetics 15:299. (ICFC, IP, CMCD) 2. Katoh S, et al. 1994. J. Immunol. 153:3440. (ELISA) 3. Budd RC, et al. 1987. J. Immunol. 138:3120. (IP) 4. Camp RL, et al. 1993. J. Exp. Med. 178:497. (Block) 5. Weiss JM, et al. 1997. J. Cell Biol. 137:1137. (Block) 6. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC) PubMed 7. Cuff CA, et al. 2001. J. Clin. Invest. 108:1031. (IHC) 8. Lee JW, et al. 2006. Nature Immunol. 8:181. 9. Zhang N, et al. 2005. J. Immunol. 174:6967. PubMed 10. Huabiao C, et al. 2005. J. Immunol. 175:591. PubMed 11. Gui J, et al. 2007. Int. Immunol. 19:1201. PubMed 12. Wang XY, et al. 2008. Blood 111:2436. PubMed 13. Kenna TJ, et al. 2008. Blood 111:2091. PubMed 14. Yamazaki J, et al. 2009. Blood PubMed 15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed 16. Chen YW, et al. 2010. Mol. Cancer Ther. 9:2879. PubMed 17. Zheng Z, et al. 1995. J. Cell. Biol. 130:485. 18. Wiranowska M, et al. 2010. Int. J. Cancer 127:532. "
Description	CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.
Storage	The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.