Purified anti-H2A.X Phospho (Ser139)_详细资料

产品信息

克隆性：多克隆保存条件：蓝冰规格： 100 ug

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Purified anti-H2A.X Phospho (Ser139)参数介绍>>>>

Clone 2F3
Isotype Mouse IgG1, κ
Reactivity Human
Immunogen Modified peptide
Formulation This H2A.X antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation The antibody was purified by affinity chromatography.
Concentration 0.5mg/ml
Recommended Usage
Each lot of this H2A.X antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1~4 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
"
Applications
IF, WB, IHC, ICFC
Application Notes
Intracellular staining protocol for Anti-H2A.X-Phosphorylated (Ser139) Antibody for Flow Cytometry

1. Prepare 70% absolute ethanol. Chill solution by storing at -20C.
2. Prepare cells of interest.
3. Wash 1X: resuspend with PBS, then pellet cells by centrifugation (250Xg, 5min)
4. Discard the supernatant and vortex to loosen cell pellet.
5. Add pre-cooled 70% ethanol drop by drop, while vortexing.
6. Incubate at -20C for 60 minutes.
7. Wash 3X with BioLegend Cell Staining Buffer and resuspend the cells at 0.5-1 X 10^7/ml in the cell staining buffer.
8. Perform immunofluorescent staining.
Additional reported applications (for the relevant formats of this clone) include: immunohistochemistry on paraffin embedded sections², immunofluorescence microscopy^3-9, western blot ^10-12, and flow cytometry^1,13.
"
Application References
1. Moiseenko V, et al. 2008 Radiat Oncol 3:18 (FC)
2. Akbay A,et al. 2008. Am J Pathol. 173:536. (IHC) PubMed
3. Mochizuki K, et al.2008.J cell Sci.121:2148. (IF) PubMed
4. Xiao R, et al. 2007. Mol Cell Biol.27:5393. (IF) PubMed
5. Rossi DJ, et al. 2007. Nature. 447:725. (IF) PubMed
6. Loidl J, et al. 2009. Mol Cell Biol. 20:2048. (IF) PubMed
7. Beels L, et al. 2009. Circulation. 120:1903. (IF) PubMed
8. Suzuki K, et al. 2010. Nucleic Acids Res. 38:e129. (IF) PubMed.
9. Lukaszewicz A. 2010. Chromasoma Apr 27. [Epub ahead of print] (IF) PubMed
10. Yamada C, et al. 2010 J. Biol. Chem. 285:16693. (WB) PubMed
11. Bu Y, et al. 2010, Biochem Biophys Res Commun. 397:157. (WB) PubMed
12. Massignan T, et al. 2010. J. Biol Chem. 285:7752. (WB) PubMed.
13. Banath JP, et al. 2010. BMC Cancer 10:4 (FC)
14. Zhang M., et al. 2011. Cancer Res. 23:7155. PubMed.
15. Kuefner MA, et al. 2012. Radiology 264:59. PubMed.
16. Yoshihara Y, et al. 2012. Biochem Biophys Res Cmmun. 421:57. PubMed.
"
Description
H2A.X is a 14 kD basal histone and a member of the H2 histone family. This nuclear protein is synthesized in the G1 and S phase of the cell cycle and is known to be important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 after double-stranded DNA breaks. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability. The 2F3 monoclonal antibody reacts with phosphorylated human H2A.X (Ser139) and has been shown to be useful for Western blotting and immunofluorescence.
"
Storage Upon receipt, store at 4°C.

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Purified anti-H2A.X Phospho (Ser139)

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Clone	2F3
Isotype	Mouse IgG1, κ
Reactivity	Human
Immunogen	Modified peptide
Formulation	This H2A.X antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation	The antibody was purified by affinity chromatography.
Concentration	0.5mg/ml
Recommended Usage	Each lot of this H2A.X antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1~4 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application. "
Applications	IF, WB, IHC, ICFC
Application Notes	Intracellular staining protocol for Anti-H2A.X-Phosphorylated (Ser139) Antibody for Flow Cytometry 1. Prepare 70% absolute ethanol. Chill solution by storing at -20C. 2. Prepare cells of interest. 3. Wash 1X: resuspend with PBS, then pellet cells by centrifugation (250Xg, 5min) 4. Discard the supernatant and vortex to loosen cell pellet. 5. Add pre-cooled 70% ethanol drop by drop, while vortexing. 6. Incubate at -20C for 60 minutes. 7. Wash 3X with BioLegend Cell Staining Buffer and resuspend the cells at 0.5-1 X 10^7/ml in the cell staining buffer. 8. Perform immunofluorescent staining. Additional reported applications (for the relevant formats of this clone) include: immunohistochemistry on paraffin embedded sections², immunofluorescence microscopy^3-9, western blot ^10-12, and flow cytometry^1,13. "
Application References	1. Moiseenko V, et al. 2008 Radiat Oncol 3:18 (FC) 2. Akbay A,et al. 2008. Am J Pathol. 173:536. (IHC) PubMed 3. Mochizuki K, et al.2008.J cell Sci.121:2148. (IF) PubMed 4. Xiao R, et al. 2007. Mol Cell Biol.27:5393. (IF) PubMed 5. Rossi DJ, et al. 2007. Nature. 447:725. (IF) PubMed 6. Loidl J, et al. 2009. Mol Cell Biol. 20:2048. (IF) PubMed 7. Beels L, et al. 2009. Circulation. 120:1903. (IF) PubMed 8. Suzuki K, et al. 2010. Nucleic Acids Res. 38:e129. (IF) PubMed. 9. Lukaszewicz A. 2010. Chromasoma Apr 27. [Epub ahead of print] (IF) PubMed 10. Yamada C, et al. 2010 J. Biol. Chem. 285:16693. (WB) PubMed 11. Bu Y, et al. 2010, Biochem Biophys Res Commun. 397:157. (WB) PubMed 12. Massignan T, et al. 2010. J. Biol Chem. 285:7752. (WB) PubMed. 13. Banath JP, et al. 2010. BMC Cancer 10:4 (FC) 14. Zhang M., et al. 2011. Cancer Res. 23:7155. PubMed. 15. Kuefner MA, et al. 2012. Radiology 264:59. PubMed. 16. Yoshihara Y, et al. 2012. Biochem Biophys Res Cmmun. 421:57. PubMed. "
Description	H2A.X is a 14 kD basal histone and a member of the H2 histone family. This nuclear protein is synthesized in the G1 and S phase of the cell cycle and is known to be important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 after double-stranded DNA breaks. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability. The 2F3 monoclonal antibody reacts with phosphorylated human H2A.X (Ser139) and has been shown to be useful for Western blotting and immunofluorescence. "
Storage	Upon receipt, store at 4°C.