Pacific Blue™ anti-human Granzyme A_详细资料

产品信息

克隆性：多克隆保存条件：蓝冰规格： 25 ug

上海恒斐生物经销国内外知名品Pai试剂抗体，折扣低，货期快，欢迎新老客户咨询订购！
品Pai介绍：Biolegend公司自2002年成立以来，一直致力于为生物医药研究领域提供YL的，高质量的流式抗体、试剂盒及其它相关产品。公司聚集了一批来自PharMingen的专业杰出人士，建立了一支雄厚技术背景的专业队伍，并通过其自身的先天优势，以及与世界知名实验室和研究所的良好合作关系，以最合理的价格为广大客户提供ZY的免疫学和细胞生物学产品，并不断地开发新产品，以满足飞速发展的生命科学研究的需要。短短几年时间，Biolegend公司得到了飞速发展，已跻身ZX进的抗体公司行列，并在2009年成为美国哈佛大家的抗体供应商！Biolegend公司的试剂主要包括细胞免疫、细胞因子、趋化因子、粘附分子等及与癌症研究、T 调节细胞研究、干细胞研究、先天免疫研究、细胞周期分析、凋亡研究及特定修饰类抗体等领域相关的试剂，用于流式、ELISA、免疫沉淀、WB、免疫荧光、免疫组化、及体内体外功能学试验。其中，Biolegend公司的流式抗体以其具有较宽范围的荧光选择及较高的性价比，为广大科研工作者所接受！同时，公司配备了一支经验丰富的技术服务队伍，为客户提供WM的配色方案及快速的售后处理服务！
Pacific Blue™ anti-human Granzyme A参数介绍>>>>

Clone CB9
Isotype Mouse IgG1, κ
Reactivity Human
Immunogen Purified human Granzyme A
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration 0.5mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 10⁶ cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Applications
ICFC - Quality tested
Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining³ of formalin-fixed paraffin-embedded tissue sections, and immunoprecipitation²
.
Application References
1. Trimble L, et al. 1998. Blood 91:585.
2. Beresford P, et al. 1997. P. Natl. Acad. Sci. USA 94:9285.
3. Raqib R, et al. 2002. Infect. Immun. 70:3199.
4. Chen H, et al. 2005. J. Immunol. 175:591.
5. Cellerai C, et al. 2010. J. Virol. 84:3868. PubMed
6. Yamamoto T, et al. 2012. J Virol. 86:5877. PubMed.
"
Description
Granzyme A is a 28 kD disulfide-linked homodimeric protein and the most abundant of the proteases occurring in CTL granules. It is homologous to other serine esterases, including other granyzmes, mast cell proteases, and neutrophil cathepsins. Granzyme B is thought to be a rapidly-acting apoptotic enzyme, while Granzyme A is slow acting. The CB9 monoclonal antibody recognizes human Granzyme A and has been shown to be useful for flow cytometry, immunoprecipitation, and immunohistochemistry (paraffin-embedded sections).
Storage The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

您可能感兴趣的产品

Pacific Blue™ anti-human Granzyme A

产品信息

联系我们

关注我们

Clone	CB9
Isotype	Mouse IgG1, κ
Reactivity	Human
Immunogen	Purified human Granzyme A
Formulation	Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation	The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration	0.5mg/ml
Recommended Usage	Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 10⁶ cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application. * Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Applications	ICFC - Quality tested
Application Notes	Additional reported applications (for the relevant formats) include: immunohistochemical staining³ of formalin-fixed paraffin-embedded tissue sections, and immunoprecipitation² .
Application References	1. Trimble L, et al. 1998. Blood 91:585. 2. Beresford P, et al. 1997. P. Natl. Acad. Sci. USA 94:9285. 3. Raqib R, et al. 2002. Infect. Immun. 70:3199. 4. Chen H, et al. 2005. J. Immunol. 175:591. 5. Cellerai C, et al. 2010. J. Virol. 84:3868. PubMed 6. Yamamoto T, et al. 2012. J Virol. 86:5877. PubMed. "
Description	Granzyme A is a 28 kD disulfide-linked homodimeric protein and the most abundant of the proteases occurring in CTL granules. It is homologous to other serine esterases, including other granyzmes, mast cell proteases, and neutrophil cathepsins. Granzyme B is thought to be a rapidly-acting apoptotic enzyme, while Granzyme A is slow acting. The CB9 monoclonal antibody recognizes human Granzyme A and has been shown to be useful for flow cytometry, immunoprecipitation, and immunohistochemistry (paraffin-embedded sections).
Storage	The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.